中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1076-1081.doi: 10.4103/1673-5374.160097

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

黄体酮的神经保护作用:减轻脑缺血后的脑水肿

  

  • 收稿日期:2015-06-10 出版日期:2015-07-24 发布日期:2015-07-24
  • 基金资助:

    基金资助:国家自然科学基金(81301006)

Progesterone is neuroprotective by inhibiting cerebral edema after ischemia

Yuan-zheng Zhao, Min Zhang, Heng-fang Liu, Jian-ping Wang*   

  1. Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China
  • Received:2015-06-10 Online:2015-07-24 Published:2015-07-24
  • Contact: Jian-ping Wang, M.D., jianpwang@126.com.
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China, No. 81301006; a grant from Henan Provincial Scientific and Technological Research Projects of China, No. 132102310092.

摘要:

缺血性脑水肿可引起血脑屏障通透性及结构改变。近期研究表明黄体酮能显著减少脑缺血后脑水肿,然而其确切机制尚不明确,为了明确其作用机制,我们采用脑组织伊文思蓝染色、RT-PCR和Western blot检测,见黄体酮能有效减少大鼠脑缺血后48 h缺血半暗带内伊文思蓝漏出量,而对缺血核心区的漏出量无影响,能抑制大鼠缺血脑组织中血脑屏障紧密连接蛋白-封闭蛋白和紧密连接蛋白1的表达下调。实验结果证实,黄体酮可有效抑制脑缺血后密连接蛋白的表达下调,从而保持血脑屏障的完整性,达到减轻脑水肿的效果。

关键词: 神经再生, 脑损伤, 性腺激素, 脑缺血, 渗透, 封闭蛋白, 紧密连接蛋白1, 伊文思蓝, 半暗带, 缺血核心, 大鼠, 国家自然科学基金

Abstract:

Ischemic edema can alter the structure and permeability of the blood-brain barrier. Recent studies have reported that progesterone reduces cerebral edema after cerebral ischemia. However, the underlying mechanism of this effect has not yet been elucidated. In the present study, progesterone effectively reduced Evans blue extravasation in the ischemic penumbra, but not in the ischemic core, 48 hours after cerebral ischemia in rats. Progesterone also inhibited the down-regulation of gene and protein levels of occludin and zonula occludens-1 in the penumbra. These results indicate that progesterone may effectively inhibit the down-regulation of tight junctions, thereby maintaining the integrity of the blood-brain barrier and reducing cerebral edema.

Key words: nerve regeneration, brain injury, gonadal hormone, cerebral ischemia, permeability, occludin, zonula occludens-1, Evans blue dye, penumbra, ischemic core, rats, neural regeneration