中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1317-1320.doi: 10.4103/1673-5374.162766

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

三七总皂苷保护横断伤脊髓与上调神经营养因子有关

  

  • 收稿日期:2015-07-11 出版日期:2015-08-24 发布日期:2015-08-24

Panax notoginseng saponins improve recovery after spinal cord transection by upregulating neurotrophic factors

Bo Wang, Yu Li, Xuan-peng Li, Yang Li   

  1. Department of Neurosurgery, First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan Province, China
  • Received:2015-07-11 Online:2015-08-24 Published:2015-08-24
  • Contact: Yu Li, liyu650410@sina.com.

摘要:

中药三七的有效活性成分三七总皂苷可保护受损神经元,但其机制尚不清楚。为此,实验建立T10脊髓横断损伤模型大鼠,损伤后30min尾静脉注射100mg/kg三七总皂苷,发现损伤后7-30d,三七总皂苷治疗的大鼠BBB评分增加。且损伤后7d,大鼠脊髓损伤节段前角中神经生长因子和脑源性神经营养因子免疫阳性神经元增加。表明三七总皂苷可上调横断损伤脊髓中神经生长因子和脑源性神经营养因子的表达,改善大鼠后肢运动功能。

关键词: 神经再生, 三七总皂苷, 脊髓损伤, 神经生长因子, 脑源性神经营养因子, 中药

Abstract:

Saponins extracted from Panax notoginseng are neuroprotective, but the mechanisms underlying this effect remain unclear. In the present study, we established a rat model of thoracic (T10) spinal cord transection, and injected Panax notoginseng saponins (100 mg/kg) or saline 30 minutes after injury. Locomotor functions were assessed using the Basso, Beattie, and Bresnahan (BBB) scale from 1 to 30 days after injury, and immunohistochemistry was carried out in the ventral horn of the spinal cord at 1 and 7 days to determine expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Our results show that at 7–30 days post injury, the BBB score was higher in rats treated with Panax notoginseng saponins than in those that received saline. Furthermore, at 7 days, more NGF- and BDNF-immunoreactive neurons were observed in the ventral horn of the spinal cord of rats that had received Panax notoginseng saponins than in those that received saline. These results indicate that Panax notoginseng saponins caused an upregulation of NGF and BDNF in rats with spinal cord transection, and improved hindlimb motor function.

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