中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (9): 1463-1470.doi: 10.4103/1673-5374.165517

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

前庭神经环路神经可塑性的可能作用

  

  • 收稿日期:2015-07-20 出版日期:2015-09-28 发布日期:2015-09-28
  • 基金资助:

    匈牙利科学院资助项目；匈牙利欧盟与the State联合欧洲社会基金框架项目

Modification of tenascin-R expression following unilateral labyrinthectomy in rats indicates its possible role in neural plasticity of the vestibular neural circuit

Botond Gaal1, Einar Örn Jóhannesson1, Amit Dattani1, Agnes Magyar2, Ildikó Wéber1, Clara Matesz1, 3   

  1. 1 Department of Anatomy, Histology and Embryology, Faculty of Medicine and Faculty of Dentistry, University of Debrecen, Nagyerdei krt. 98, Debrecen, Hungary
    2 Department of Pediatric Surgery, Faculty of Medicine, Clinical Center, University of Debrecen, Nagyerdei krt. 98, Debrecen, Hungary
    3 MTA-DE Neuroscience Research Group, University of Debrecen, Nagyerdei krt. 98, Debrecen, Hungary
  • Received:2015-07-20 Online:2015-09-28 Published:2015-09-28
  • Contact: Clara Matesz, M.D., Ph.D., matesz@anat.med.unideb.hu.
  • Supported by:

    This work was supported by a grant from the Hungarian Academy of Sciences (MTA-TKI 11008); a grant from the European Union and the State of Hungary; and the European Social Fund in the framework of TÁMOP-4.2.4.A/ 2-11/1-2012-0001 ‘National Excellence Program’

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摘要：

既往研究显示，大鼠单侧迷路切除伴随前庭外侧核透明质酸和软骨素蛋白聚糖染色的改变，后续的细胞外基质重组时间进程与前庭功能障碍的恢复有关。细胞外基质糖蛋白--腱糖蛋白R对轴突生长和再生及神经环路的修复有抑制作用。实验应用免疫组化方法研究了大鼠行单侧迷路切除后，腱糖蛋白R在前庭神经上核、内侧核、外侧核、降核中的表达。切除后第1天，迷路切除侧前庭神经上核、内侧核、外侧核、降核喙部围神经元网未见腱糖蛋白R免疫反应。第3天，腱糖蛋白R免疫反应在切除侧前庭神经内侧核围神经元网中有所恢复，至第7天，其免疫反应在前庭神经上核、外侧核、降核喙部有所恢复。但在前庭神经降核尾部，腱糖蛋白R免疫反应则一直未见有所改变。腱糖蛋白R表达的分布差异可能与前庭神经核各部在代偿过程中的作用不同有关。tenascin-R表达的下降可能提示迷路损伤后的前庭神经环路可塑性的细胞外易化。

关键词: 细胞外基质, 脑干, 前庭系统, 前庭损害, 前庭代偿, 围神经元网, 神经可塑性

Abstract:

We have previously found that unilateral labyrinthectomy is accompanied by modification of hyaluronan and chondroitin sulfate proteoglycan staining in the lateral vestibular nucleus of rats and the time course of subsequent reorganization of extracellular matrix assembly correlates to the restoration of impaired vestibular function. The tenascin-R has repelling effect on pathfinding during axonal growth/regrowth, and thus inhibits neural circuit repair. By using immunohistochemical method, we studied the modification of tenascin-R expression in the superior, medial, lateral, and descending vestibular nuclei of the rat following unilateral labyrinthectomy. On postoperative day 1, tenascin-R reaction in the perineuronal nets disappeared on the side of labyrinthectomy in the superior, lateral, medial, and rostral part of the descending vestibular nuclei. On survival day 3, the staining intensity of tenascin-R reaction in perineuronal nets recovered on the operated side of the medial vestibular nucleus, whereas it was restored by the time of postoperative day 7 in the superior, lateral and rostral part of the descending vestibular nuclei. The staining intensity of tenascin-R reaction remained unchanged in the caudal part of the descending vestibular nucleus bilaterally. Regional differences in the modification of tenascin-R expression presented here may be associated with different roles of individual vestibular nuclei in the compensatory processes. The decreased expression of the tenascin-R may suggest the extracellular facilitation of plastic modifications in the vestibular neural circuit after lesion of the labyrinthine receptors.

Key words: nerve regeneration, extracellular matrix, brainstem, vestibular system, vestibular lesion, vestibular compensation, perineuronal net, neural plasticity, neural regeneration