中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1846-1855.doi: 10.4103/1673-5374.170315

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

超声引导增强神经生长因子对损伤坐骨神经的神经保护作用

  

  • 收稿日期:2015-07-20 出版日期:2015-12-07 发布日期:2015-12-07
  • 基金资助:

    国家自然科学基金(81100922)

Neuroprotective effects of ultrasound-guided nerve growth factor injections after sciatic nerve injury

Hong-fei Li, Yi-ru Wang, Hui-ping Huo, Yue-xiang Wang*, Jie Tang*   

  1. Department of Ultrasound, Chinese PLA General Hospital, Beijing, China
  • Received:2015-07-20 Online:2015-12-07 Published:2015-12-07
  • Contact: Jie Tang, M.D. or Yue-xiang Wang, M.D., txiner@vip.sina.com or wangyuexiang1999@sina.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81100922.

摘要:

神经生长因子是促进神经再生的重要神经营养因子,但其存在半衰期短的缺陷,直接影响其作用效果。因此探寻一种能够提高给药效率的方法显得尤为重要。高频超声能够示踪周围神经,判断神经的损伤位置,引导药物直达目标区域。实验假设超声引导能增强神经生长因子对坐骨神经损伤的神经保护效应。行为学评分、超声、电生理、组织学和免疫组化染色结果显示,坐骨神经损伤后立即在超声引导下注射神经生长因子的神经保护作用优于伤后14天干预和肌肉注射神经生长因子。提示在超声引导的辅助下神经生长因子对周围神经损伤神经保护作用效能得以提高,且给药时机应趁早。

关键词: 神经再生, 高频超声, 周围神经损伤, 神经生长因子, 坐骨神经, 神经营养因子, 肌肉注射, 给药方法, 给药时机

Abstract:

Nerve growth factor (NGF) plays an important role in promoting neuroregeneration after peripheral nerve injury. However, its effects are limited by its short half-life; it is therefore important to identify an effective mode of administration. High-frequency ultrasound (HFU) is increasingly used in the clinic for high-resolution visualization of tissues, and has been proposed as a method for identifying and evaluating peripheral nerve damage after injury. In addition, HFU is widely used for guiding needle placement when administering drugs to a specific site. We hypothesized that HFU guiding would optimize the neuroprotective effects of NGF on sciatic nerve injury in the rabbit. We performed behavioral, ultrasound, electrophysiological, histological, and immunohistochemical evaluation of HFU-guided NGF injections administered immediately after injury, or 14 days later, and compared this mode of administration with intramuscular NGF injections. Across all assessments, HFU-guided NGF injections gave consistently better outcomes than intramuscular NGF injections administered immediately or 14 days after injury, with immediate treatment also yielding better structural and functional results than when the treatment was delayed by 14 days. Our findings indicate that NGF should be administered as early as possible after peripheral nerve injury, and highlight the striking neuroprotective effects of HFU-guided NGF injections on peripheral nerve injury compared with intramuscular administration.