中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 101-106.doi: 10.4103/1673-5374.175053

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

毛蕊花糖苷可促进脑黑质中酪氨酸羟化酶神经元的再生

  

  • 收稿日期:2015-10-22 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    国家自然科学基金(30672684, 30973722)

Verbascoside promotes the regeneration of tyrosine hydroxylase-immunoreactive neurons in the substantia nigra

Jian-qing Liang, Li Wang, Jian-cheng He, Xian-dong Hua   

  1. Shanghai University of Traditional Chinese Medicine, Shanghai, China
  • Received:2015-10-22 Online:2016-01-15 Published:2016-01-15
  • Contact: Jian-cheng He, M.D., hejc8163@163.com.
  • Supported by:

    This study was supported by a grant from Science and Technology Support Traditional Chinese Drug Research and Development Project
    of Shanghai of China, No. 12401900302; Traditional Chinese Medicine Research Foundation of Shanghai Municipal Health Bureau of China, No. 2012J009A; a grant from Annual Research Budget of Shanghai University of Traditional Chinese Medicine of China in 2013, No. 2013JW25; the National Natural Science Foundation of China, No. 30672684, 30973722.

摘要:

酪氨酸羟化酶是多巴胺生物合成途径的关键酶,因此酪氨酸羟化酶的表达变化与帕金森病的发生发展密切相关。从熟地黄中提取的中药单体毛蕊花糖苷已被应用于临床治疗帕金森病有效,但作用机制仍不清楚。实验假设毛蕊花糖苷治疗帕金森病的机制与酪氨酸羟化酶的变化有关。为此建立了帕金森病模型大鼠,用毛蕊花糖苷60 mg/kg,1次/d,灌胃6周。结果显示,帕金森病模型大鼠旋转行为症状减轻,大鼠脑右侧黑质酪氨酸羟化酶mRNA、蛋白表达及免疫反应阳性反应增强。结果证实,毛蕊花糖苷缓解帕金森病的机制与促进黑质中酪氨酸羟化酶神经元再生相关。

关键词: 神经再生, 中医药, 神经退行性变, 帕金森病, 运动障碍, 酪氨酸羟化酶, 神经行为, 毛蕊花糖苷, 大鼠, 国家自然科学基金

Abstract:

Tyrosine hydroxylase is a key enzyme in dopamine biosynthesis. Change in tyrosine hydroxylase expression in the nigrostriatal system is closely related to the occurrence and development of Parkinson’s disease. Verbascoside, an extract from Radix Rehmanniae Praeparata has been shown to be clinically effective in treating Parkinson’s disease. However, the underlying mechanisms remain unclear. It is hypothesized that the effects of verbascoside on Parkinson’s disease are related to tyrosine hydroxylase expression change in the nigrostriatal system. Rat models of Parkinson’s disease were established and verbascoside (60 mg/kg) was administered intraperitoneally once a day. After 6 weeks of verbascoside treatment, rat rotational behavior was alleviated; tyrosine hydroxylase mRNA and protein expression and the number of tyrosine hydroxylase-immunoreactive neurons in the rat right substantia nigra were significantly higher than the Parkinson’s model group. These findings suggest that the mechanism by which verbascoside treats Parkinson’s disease is related to the regeneration of tyrosine hydroxylase-
immunoreactive neurons in the substantia nigra.

Key words: nerve regeneration, traditional Chinese medicine, Parkinson’s disease, rats, dyskinesia, tyrosine hydroxylase, neurological behavior, verbascoside, neural regeneration