中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (2): 278-284.doi: 10.4103/1673-5374.177736

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

毛冬青总黄酮与骨髓干细胞动员对脑缺血再灌注损伤的神经保护

  

  • 收稿日期:2015-07-08 出版日期:2016-02-15 发布日期:2016-02-15
  • 基金资助:

    国家“重大新药创制”科技重大专项(2009ZX09103-324);河南省高校科技创新团队(2012IRTSTHN011)。

Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Ming-san Miao, Lin Guo, Rui-qi Li, Xiao Ma   

  1. Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
  • Received:2015-07-08 Online:2016-02-15 Published:2016-02-15
  • Contact: Ming-san Miao, M.D.,miaomingsan@126.com.
  • Supported by:

    This study was supported by a grant from the State “Major New Drug Creation” Science and Technology Major Project in China, No. 2009ZX09103-324; a grant from the Henan Provincial Science and Technology Innovation Team in University in China, No. 2012IRTSTHN011.

摘要:

作者前期研究证实毛冬青总黄酮有神经保护作用,但与人重组粒细胞集落刺激因子动员的骨髓干细胞移植治疗脑缺血再灌注损伤,是否有协同作用?实验以0.3,0.15,0.075 g/kg毛冬青总黄酮给动物模型造模前3d至损伤后第2天灌胃。结果发现毛冬青总黄酮可使脑缺血再灌注大鼠缺血脑组织病理损伤减轻,尼氏小体增多,Bax蛋白表达降低,Bcl-2蛋白表达提高,CD34阳性细胞表达增多。由此认为,毛冬青总黄酮可提高骨髓干细胞动员效果,增强神经细胞抗凋亡能力,对大鼠脑缺血再灌注损伤起神经保护作用。

关键词: 神经再生, 毛冬青总黄酮, 骨髓干细胞, 动员, 脑缺血再灌注, 尼氏小体, Bax, Bcl-2, CD34, 神经元

Abstract:

Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids
could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective
effect on cerebral ischemia/reperfusion injury in rats.

Key words: nerve regeneration, Radix Ilicis Pubescentis total flavonoids, bone marrow stem cells, mobilization, cerebral ischemia/reperfusion, Nissl bodies, Bax, Bcl-2, CD34, neurons, neural regeneration