中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (7): 1147-1152.doi: 10.4103/1673-5374.187055

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Ca2+依赖酸性磷酸酶活性检测鉴定细菌黑色素干预后损伤中枢神经的完整性

  

  • 出版日期:2016-07-30 发布日期:2016-07-30
  • 基金资助:
    亚美尼亚国家自然教育基金

Detection of Ca2+-dependent acid phosphatase activity identifies neuronal integrity in damaged rat central nervous system after application of bacterial melanin.

Tigran R. Petrosyan1, *, Anna S. Ter-Markosyan1, Anna S. Hovsepyan2   

  1. 1 Yerevan State Medical University, Yerevan, Armenia 2 SPC “Armbiotechnology” SNPO NAS RA, Yerevan, Armenia
  • Online:2016-07-30 Published:2016-07-30
  • Contact: Tigran R. Petrosyan, Ph.D., tigpetrosyan@mail.ru.
  • Supported by:
    This study was supported by the Armenian National Science and Education Fund for Project in New York, USA (No. ANSEF biotech-4241).

摘要:

实验应用Ca2+依赖酸性磷酸酶活性检测,试图证实细菌黑色素对中枢神经系统损伤的神经保护作用。纳入24只大鼠随机等分为2组,分别给予单侧感觉运动皮质损毁和颈髓水平红核脊髓束横断。每组各有6只大鼠术后肌肉注射细菌黑色素溶液,其余6只大鼠注射生理盐水作为对照组。神经功能评分显示,细菌黑色素对2组大鼠的运动功能恢复均有加速作用。损伤后2个月,Ca2+依赖酸性磷酸酶活性结合Chilingarian钙ATP法检测显示,细菌黑色素刺激神经纤维生长且扩张大脑和脊髓毛细胞血管。说明细菌黑色素对中枢神经系统损伤后运动功能的恢复有促进作用;Ca2+依赖酸性磷酸酶活性检测可有效评估细菌黑色素促进损伤中枢神经系统再生作用。 

orcid: 0000-0002-5517-8856 (Tigran R. Petrosyan)

关键词: 神经再生, 细菌黑色素, 组织化学染色, 红核脊髓束, 感觉运动皮质, Ca2+依赖酸性磷酸酶活性, 大鼠

Abstract: The study aims to confirm the neuroregenerative effects of bacterial melanin (BM) on central nervous system injury using a special staining method based on the detection of Ca2+-dependent acid phosphatase activity. Twenty-four rats were randomly assigned to undergo either unilateral destruction of sensorimotor cortex (group I; n = 12) or unilateral rubrospinal tract transection at the cervical level (C3–4) (group II; n = 12). In each group, six rats were randomly selected after surgery to undergo intramuscular injection of BM solution (BM subgroup) and the remaining six rats were intramuscularly injected with saline (saline subgroup). Neurological testing confirmed that BM accelerated the recovery of motor function in rats from both BM and saline subgroups. Two months after surgery, Ca2+-dependent acid phosphatase activity detection in combination with Chilingarian’s calcium adenoside triphosphate method revealed that BM stimulated the sprouting of fibers and dilated the capillaries in the brain and spinal cord. These results suggest that BM can promote the recovery of motor function of rats with central nervous system injury; and detection of Ca2+-dependent acid phosphatase activity is a fast and easy method used to study the regeneration- promoting effects of BM on the injured central nervous system.

Key words: nerve regeneration, bacterial melanin, histochemical analysis, rubrospinal tract, sensorimotor cortex, Ca2+-dependent acid phosphatase activity, rats, neural regeneration