中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (9): 1431-1437.doi: 10.4103/1673-5374.191216

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

13-甲基肉豆蔻酸可减轻脑缺血再灌注损伤

  

  • 收稿日期:2016-08-12 出版日期:2016-09-30 发布日期:2016-09-30
  • 基金资助:
    福建省科技项目（2010y0027）；福建医科大学教育发展基金（js12001）

13-Methyltetradecanoic acid mitigates cerebral ischemia/reperfusion injury

Juan Yu1, *, Li-nan Yang1, Yan-yun Wu1, Bao-hua Li1, Sheng-mei Weng2, Chun-lan Hu1, Yong-ling Han1   

  1. 1 Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China 2 Department of Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province, China
  • Received:2016-08-12 Online:2016-09-30 Published:2016-09-30
  • Contact: Juan Yu, tune9@163.com.
  • Supported by:
    This research was supported by grants from of the Science and Technology Project of Fujian Province of China, No. 2010Y0027; the Professor Development Fund of Fujian Medical University of China, No. JS12001.

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摘要：

13-甲基肉豆蔻酸具有维持细胞膜的稳定性、抗炎、抗氧化及抗凋亡作用，但以往研究多以周围神经损伤修复为主，应用在中枢神经系统疾的相关研究罕见报道。我们假设13-甲基肉豆蔻酸干预局灶性脑缺血再灌注损伤可发挥神经保护作用，并观察脑组织神经再生相关因子-碱性成纤维细胞生长因子和血管内皮生长因子的表达情况。实验采用线栓法制备大鼠大脑中动脉闭塞/再灌注损伤模型，线栓栓塞缺血2 h，拔出线栓再灌注24 h，干预灌注开始时尾静脉注射13-甲基肉豆蔻酸10，40，80 mg/kg。发现再灌注24 h，13-甲基肉豆蔻酸各浓度组均能有效缩小大鼠脑梗死灶体积，减轻脑水肿，增加损伤侧脑组织碱性成纤维生长因子、血管内皮生长因子mRNA以及免疫阳性表达，以应用13-甲基肉豆蔻酸40，80 mg/kg组效果最为显著。结果证实，再灌注即刻给予13-甲基肉豆蔻酸可减轻局灶性脑缺血再灌注损伤，同时刺激碱性成纤维生长因子、血管内皮生长因子的表达上调，以此来更好地发挥神经保护作用。 

orcid: 0000-0002-7264-6463 (Juan Yu)

关键词: 神经再生, 脑损伤, 13-甲基十四烷酸, 脑缺血, 再灌注损伤, 碱性成纤维生长因子, 血管内皮生长因子, 大脑中动脉, 脑梗死灶, 脑水肿, 血脑屏障, 大鼠

Abstract: 13-Methyltetradecanoic acid can stabilize cell membrane and have anti-in?ammatory, antioxidant and anti-apoptotic effects. Previous studies mainly focused on peripheral nerve injury, but seldom on the central nervous system. We investigated whether these properties of 13-methyltetradecanoic acid have a neuroprotective effect on focal cerebral ischemia/reperfusion injury, and detected the expression of basic fbroblast growth factor and vascular endothelial growth factor. This study established rat models of middle cerebral artery occlusion/ reperfusion injury by ischemia for 2 hours and reperfusion for 24 hours. At the beginning of reperfusion, 13-methyltetradecanoic acid 10, 40 or 80 mg/kg was injected into the tail vein. Results found that various doses of 13-methyltetradecanoic acid effectively reduced infarct volume, mitigate cerebral edema, and increased the mRNA and protein expression of basic fbroblast growth factor and vascular endothelial growth factor at 24 hours of reperfusion. The effect was most signifcant in the 13-methyltetradecanoic acid 40 and 80 mg/kg groups. The fndings suggest that 13-methyltetradecanoic acid can relieve focal ischemia/reperfusion injury immediately after reperfusion, stimulate the upregulation of basic fbroblast growth factor and vascular endothelial growth factor to exert neuroprotective effects.

Key words: nerve regeneration, brain injury, 13-methyltetradecanoic acid, cerebral ischemia/reperfusion injury, basic fibroblast growth factor, vascular endothelial growth factor, cerebral infarction, cerebral edema, neural regeneration