中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (10): 1610-1616.doi: 10.4103/1673-5374.193240

• 原著:退行性病与再生 • 上一篇    下一篇

羟基柠檬酸干预减轻多发性硬化小鼠炎症和氧化应激反应的神经保护作用

  

  • 收稿日期:2016-09-18 出版日期:2016-10-31 发布日期:2016-10-31
  • 基金资助:
    伊朗Alborz大学医学院基金

Hydroxycitric acid ameliorates in?ammation and oxidative stress in mouse models of multiple sclerosis

Mahdi Goudarzvand1, Sanaz Afraei2, *, Somaye Yaslianifard3, Saleh Ghiasy4, Ghazal Sadri4, Mustafa Kalvandi1, Tina Alinia4, Ali Mohebbi5, Reza Yazdani6, Shahin Khadem Azarian2, Abbas Mirshafey2, Gholamreza Azizi7, 4, *   

  1. 1 Department of Physiology and Pharmacology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran 2 Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Microbiology and Immunology, School of Medicine,Alborz University of Medical Sciences, Karaj, Iran 4 Research Centre for Immunodefciencies, Pediatrics Centre of Excellence, Children’s Medical Centre, Tehran University of Medical Sciences, Tehran, Iran 5 Growth and Development Research Centre, Paediatrics Centre of Excellence, Children’s Medical Centre, Tehran University of Medical Sciences, Tehran, Iran 6 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 7 Department of Laboratory Medicine, Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran
  • Received:2016-09-18 Online:2016-10-31 Published:2016-10-31
  • Contact: Gholamreza Azizi, Ph.D. or Sanaz Afraei, M.Sc., azizi_g@razi.tums.ac.ir or Sanaz_afraei@yahoo.com.
  • Supported by:
    This work was supported by Vice Chancellor for Research, Alborz University of Medical Sciences, No. 1394-01-01-1050.

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摘要：

羟基柠檬酸是一种柠檬酸的衍生物,主要存在于藤黄果中，其抗炎作用已引起学者广泛关注。多发性硬化会引起神经损伤的炎性脱髓鞘和轴索损害。我们假设羟基柠檬酸对多发性硬化有治疗作用，实验设计以含髓鞘少突胶质细胞糖蛋白35-55的弗氏完全佐剂乳液诱导建立一种多发性硬化动物模型，实验性自身免疫性脑脊髓炎小鼠模型，按2g/kg的剂量给予实验性自身免疫性脑脊髓炎小鼠羟基柠檬酸治疗3周，发现羟基柠檬酸干预明显改善了实验性自身免疫性脑脊髓炎小鼠的神经损伤症状，降低了血清白细胞介素6, 肿瘤坏死因子α, 一氧化氮和丙二醛水平，提高了超氧化物歧化酶和谷胱甘肽还原酶水平，结果表明羟基柠檬酸可能通过对抗炎症和氧化反应，对多发性硬化起的神经损伤起到保护和治疗作用。 

orcid: 0000-0001-5658-2511 (Gholamreza Azizi)

关键词: 神经再生, 羟基柠檬酸, 多发性硬化, 炎症, 氧化应激, 实验性自身免疫性脑脊髓炎

Abstract: Hydroxycitric acid (HCA) is derived primarily from the Garcinia plant and is widely used for its anti-in?ammatory e?ects. Multiple sclerosis can cause an in?ammatory demyelination and axonal damage. In this study, to validate the hypothesis that HCA exhibits therapeutic e?ects on multiple sclerosis, we established female C57BL/6 mouse models of multiple sclerosis, i.e., experimental autoimmune encephalomyelitis, using Complete Freund’s Adjuvant (CFA) emulsion containing myelin oligodendrocyte glycoprotein (35–55). Treatment with HCA at 2 g/kg/d for 3 weeks obviously improved the symptoms of nerve injury of experimental autoimmune encephalomyelitis mice, decreased serum interleulin-6, tumor necrosis factor alpha, nitric oxide, and malondialdehyde levels, and increased superoxide dismutase and glutathione reductase activities. Tese fndings suggest that HCA exhibits neuroprotective e?ects on multiple sclerosis-caused nerve injury through ameliorating in?ammation and oxidative stress.

Key words: nerve regeneration, hydroxycitric acid, multiple sclerosis, in?ammation, oxidative stress, experimental autoimmune encephalomyelitis, neural regeneration