中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 1940-1950.doi: 10.4103/1673-5374.197135

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针诱导脑卒中后细胞增殖相关miRNA差异表达:恢复脑血流和神经功能

  

  • 收稿日期:2016-11-16 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    中国国家自然科学基金项目(81173416)

Electroacupuncture induces acute changes in cerebral cortical miRNA profle, improves cerebral blood ?ow and alleviates neurological defcits in a rat model of stroke

Hai-zhen Zheng1, 2, #, Wei Jiang3, #, Xiao-feng Zhao1, *, Jing Du2, Pan-gong Liu2, Li-dan Chang2, Wen-bo Li2, Han-tong Hu2, Xue-min Shi1, 3   

  1. 1 VIP of Acupuncture Department, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 2 Tianjin University of Traditional Chinese Medicine, Tianjin, China 3 Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China
  • Received:2016-11-16 Online:2016-12-31 Published:2016-12-31
  • Contact: Xiao-feng Zhao, zhxf67@163.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81173416.

摘要:

有研究显示电针能改善脑卒中后脑血流量,但其是否可以改变大脑皮质细胞增殖相关miRNA表达目前尚不清楚。为此,实验对经1次电针连续波、频率2Hz、强度3mA、刺激1min,刺激人中和内关穴治疗的脑卒中大鼠进行分析。结果显示,电针能增加脑卒中大鼠脑血流量,减轻神经功能缺损,且大脑皮质中多种miRNA表达出现变化。包括miR-206-3p,miR-3473,miR-6216和miR-494-3p在内的多种miRNA参与多种信号通路,其中受影响最显著的信号通路是在脑卒中后期机体修复进程中传导细胞增殖信号的血管内皮生长因子信号通路,这些miRNAs的表达变化情况也被实时定量PCR所验证。实验结果验证了电针通过影响细胞增殖相关miRNA的差异表达,恢复脑血液供应,促进脑卒中后功能恢复的作用及机制。 

orcid: 0000-0002-1750-7364 (Xiao-feng Zhao)

关键词: 神经再生, 脑卒中, 大脑中动脉闭塞, 电针, miRNA, 脑血流量, 内关, 人中

Abstract: Electroacupuncture has been shown to improve cerebral blood ?ow in animal models of stroke. However, it is unclear whether electroacupuncture alters miRNA expression in the cortex. In this study, we examined changes in the cerebral cortical miRNA profle, cerebral blood ?ow and neurological function induced by electroacupuncture in a rat model of stroke. Electroacupuncture was performed at Renzhong (GV26) and Neiguan (PC6), with a frequency of 2 Hz, continuous wave, current intensity of 3.0 mA, and stimulation time of 1 minute. Electroacupuncture increased cerebral blood ?ow and alleviated neurological impairment in the rats. miRNA microarray profling revealed that the vascular endothelial growth factor signaling pathway, which links cell proliferation with stroke, was most signifcantly affected by electroacupuncture. Electroacupuncture induced changes in expression of rno-miR-206-3p, rno-miR-3473, rno-miR-6216 and rno-miR- 494-3p, and these changes were confrmed by quantitative real-time polymerase chain reaction. Our fndings suggest that changes in cell proliferation-associated miRNA expression induced by electroacupuncture might be associated with the improved cerebral blood supply and functional recovery following stroke.

Key words: nerve regeneration, stroke, middle cerebral artery occlusion, electroacupuncture, miRNA, cerebral blood ?ow, Neiguan (PC6), Renzhong (GV26), neural regeneration