中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 1951-1955.doi: 10.4103/1673-5374.197136

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

在体场记录可有效分析异氟醚麻醉对小鼠大脑皮质及海马的影响

  

  • 收稿日期:2016-11-28 出版日期:2016-12-31 发布日期:2016-12-31

In vivo feld recordings effectively monitor the mouse cortex and hippocampus under iso?urane anesthesia

Yi-qing Yin#, Li-fang Wang#, Chao Chen, Teng Gao, Zi-fang Zhao, Cheng-hui Li*   

  1. Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, China
  • Received:2016-11-28 Online:2016-12-31 Published:2016-12-31
  • Contact: Cheng-hui Li, M.D., chenghui_li@sina.com.

摘要:

异氟醚是一种临床上广泛使用的吸入麻醉剂,但其影响意识的具体机制尚无共识。因此,实验使用在体场记录检测异氟醚对大脑海马和皮质的影响。结果发现,1.3%,0.8%和0.4%的异氟醚对大脑海马和皮质低频段存在抑制作用,而上述3种浓度及1.7%的异氟醚对大脑海马和皮质高频段的抑制作用随异氟醚浓度的增加而增加,说明在体场记录能有效分析异氟烷麻醉对小鼠大脑海马和皮质的影响。 

orcid: 0000-0002-8531-2489 (Cheng-hui Li)

关键词: 神经再生, 神经元, 异氟醚, 膜片钳, 细胞膜, 突触反应, 吸入麻醉, 电生理

Abstract: Iso?urane is a widely used inhaled anesthetic in the clinical setting. However, the mechanism underlying its effect on consciousness is under discussion. Terefore, we investigated the effect of iso?urane on the hippocampus and cortex using an in vivo feld recording approach. Our results showed that 1.3%, 0.8%, and 0.4% iso?urane exerted an inhibitory in?uence on the mouse hippocampus and cortex. Further, high frequency bands in the cortex and hippocampus showed greater suppression with increasing iso?urane concentration. Our fndings suggest that in vivo feld recordings can monitor the effect of iso?urane anesthesia on the mouse cortex and hippocampus.

Key words: nerve regeneration, neurons, iso?urane, patch clamp, cell membrane, synaptic response, inhalational anesthesia, electrophysiology