中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 2012-2017.doi: 10.4103/1673-5374.197146

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

生物可降解镁金属丝促进坐骨神经损伤的修复

  

  • 收稿日期:2016-11-12 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    国家自然科学基金(81400528); 中国博士后科学基金(20130390827)

Biodegradable magnesium wire promotes regeneration of compressed sciatic nerves

Bo-han Li1, 2, Ke Yang3, Xiao Wang4, *   

  1. 1 Department of Oral & Maxillofacial Surgery, Te General Hospital of the People’s Liberation Army, Beijing, China 2 Department of Oral & Maxillofacial Surgery, Binzhou Medical University, Yantai, Shandong Province, China 3 Metal Research Institute of Chinese Academy of Sciences, Shenyang, Liaoning Province, China 4 Department of Oral & Maxillofacial Surgery, Peking University Tird Hospital, Beijing, China
  • Received:2016-11-12 Online:2016-12-31 Published:2016-12-31
  • Contact: Xiao Wang, bysywangxiao@163.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81400528; the China Postdoctoral Science Foundation, No. 20130390827.

摘要:

已有研究证明生物可降解镁金属丝具有良好的抗炎性和生物降解性,可促进许旺细胞分泌神经生长因子和促进中枢神经系统损伤神经轴突的再生。为此,我们假设生物可降解镁金属丝也可促进周围神经神的修复与再生。实验建立了坐骨神经硅胶管急性挤压伤模型,用生物可降解镁金属丝(直径3 mm,长8 mm)桥接于神经损伤两端,发现术后4周大鼠坐骨神经功能指数、背根神经节中神经生长因子、p75NTR及trkA mRNA表达均升高,损伤神经横断面组织神经纤维数和再生的轴突数量增多,可见坐骨神经组织再生较为明显的效果,也证明了生物可降解镁金属丝可有效促进大鼠挤压损伤坐骨神经修复的假设。

orcid: 0000-0003-3710-026X (Xiao Wang)

关键词: 神经再生, 周围神经再生, 生物可降解, 镁金属丝, 坐骨神经, 大鼠, 神经生长因子, p75NTR, trkA

Abstract: Magnesium (Mg) wire has been shown to be biodegradable and have anti-in?ammatory properties. It can induce Schwann cells to secrete nerve growth factor and promote the regeneration of nerve axons afer central nervous system injury. We hypothesized that biodegradable Mg wire may enhance compressed peripheral nerve regeneration. A rat acute sciatic nerve compression model was made, and AZ31 Mg wire (3 mm diameter; 8 mm length) bridged at both ends of the nerve. Our results demonstrate that sciatic functional index, nerve growth factor, p75 neurotrophin receptor, and tyrosine receptor kinase A mRNA expression are increased by Mg wire in Mg model. Te numbers of cross section nerve fbers and regenerating axons were also increased. Sciatic nerve function was improved and the myelinated axon number was increased in injured sciatic nerve following Mg treatment. Immuno?uorescence histopathology showed that there were increased vigorous axonal regeneration and myelin sheath coverage in injured sciatic nerve afer Mg treatment. Our fndings confrm that biodegradable Mg wire can promote the regeneration of acute compressed sciatic nerves.

Key words: nerve regeneration, peripheral nerve regeneration, biodegradable, magnesium wire, sciatic nerve, rats, nerve growth factor, P75 neurotrophin receptor, tyrosine receptor kinase A, neural regeneration