中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (2): 321-328.doi: 10.4103/1673-5374.200815

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

局灶性脑缺血非人灵长目动物模型

  

  • 收稿日期:2017-01-18 出版日期:2017-02-15 发布日期:2017-02-15
  • 基金资助:

    中国国家自然科学基金项目（81000852，81301677），AHA奖励（17POST32530004），四川省科技支撑项目（2012SZ0140），浙江省科研项目（201022896）

Nonhuman primate models of focal cerebral ischemia

Jingjing Fan1, 2, Yi Li1, 2, Xinyu Fu1, 2, Lijuan Li1, 2, Xiaoting Hao3, Shasha Li1, 2, 4, 5   

  1. 1 Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China; 
    2 Province Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China; 
    3 Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China; 
    4 Department of Psychiatry, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; 
    5 Athinoula A. Martions Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  • Received:2017-01-18 Online:2017-02-15 Published:2017-02-15
  • Contact: Shasha Li, M.D., Ph.D.,pmr.shashali@gmail.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81000852 and 81301677; the AHA Award, No. 17POST32530004; the Supporting Project of Science & Technology of Sichuan Province of China, No. 2012SZ0140; the Research Foundation of Zhejiang Province of China, No. 201022896.

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摘要：

在以往的研究中，啮齿类动物被广泛的应用于脑缺血模型的制作。但是在从动物实验到临床研究的转化过程中却鲜有阳性结果的出现。相比而言，非人灵长目动物的大脑在结构、生理、功能及神经学上与人类大脑非常相似，更能模拟出人类脑缺血损伤的过程。作者全面总结了电凝、内皮素1诱导闭塞、微血管夹闭、自体血凝块栓塞、气囊扩张、微导管栓塞、弹簧圈栓塞、外科缝合栓塞、线栓、光化学诱导共10种常用的非人灵长目大脑中动脉闭塞模型的造模方法，介绍了每种方法的优劣以及造模时的注意事项，同时对非人灵长目与啮齿类动物，非人灵长目不同种属，以及不同造模方式进行了对比，并讨论了造模时需要考虑的各种因素和造模后的评估方法，以了解他们各自的优势和劣势，以便为未来实验选择最佳模型。

ORCID:0000-0002-2498-6893(Shasha Li)

关键词: 神经再生, 脑卒中, 脑缺血, 大脑中动脉闭塞, 非人灵长目动物, 模型选择

Abstract:

Rodents have been widely used in the production of cerebral ischemia models. However, successful therapies have been proven on experimental rodent stroke model, and they have often failed to be effective when tested clinically. Therefore, nonhuman primates were recommended as the ideal alternatives, owing to their similarities with the human cerebrovascular system, brain metabolism, grey to white matter ratio and even their rich behavioral repertoire. The present review is a thorough summary of ten methods that establish nonhuman primate models of focal cerebral ischemia; electrocoagulation, endothelin-1-induced occlusion, microvascular clip occlusion, autologous blood clot embolization, balloon inflation, microcatheter embolization, coil embolization, surgical suture embolization, suture, and photochemical induction methods. This review addresses the advantages and disadvantages of each method, as well as precautions for each model, compared nonhuman primates with rodents, different species of nonhuman primates and different modeling methods. Finally it discusses various factors that need to be considered when modelling and the method of evaluation after modelling. These are critical for understanding their respective strengths and weaknesses and underlie the selection of the optimum model.

Key words: nerve regeneration, stroke, cerebral ischemia, middle cerebral artery occlusion, nonhuman primates, model selection, neural regeneration