中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (6): 925-930.doi: 10.4103/1673-5374.208574

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针调节脑缺血再灌注后的应激损伤修复链

  

  • 收稿日期:2017-05-24 出版日期:2017-06-15 发布日期:2017-06-15
  • 基金资助:

     

    国家科技重大专项课题任务——重大新药创制(2009ZX09103-707

Electroacupuncture regulates the stress-injury-repair chain of events after cerebral ischemia/reperfusion injury

Peng Shi, Lin-lin Sun, Yi-shuo Lee, Ya Tu   

  1. School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China
  • Received:2017-05-24 Online:2017-06-15 Published:2017-06-15
  • Contact: Ya Tu, tuyab@263.net.
  • Supported by:

    This work was supported by a grant from the Major Science and Technology Project “Major New Drug Created” Funding, No. 2009ZX09103-707.

摘要:

卒中后炎性反应是造成脑缺血再灌注后损伤的主要原因,损伤后受损细胞释放热休克蛋白70等内源性损伤信号,进而通过应激-损伤-修复链的级联反应可引起细胞死亡,同时也可启动修复功能发挥脑保护作用。针刺作为治疗脑缺血卒中的有效手段,是否介入了这一反应链而发挥作用呢?为验证此问题,实验首先通过Zea Longa法建立脑缺血再灌注大鼠模型,以神经功能评价、TTC染色和苏木精-伊红染色鉴定缺血再灌注损伤模型建立成功。然后以电针干预脑缺血再灌注模型大鼠百会配伍足三里,疏密波,2-100Hz,2mA,持续20min,1次/d。显示脑缺血再灌注后大鼠血清中应激损伤修复链的重要因子促肾上腺皮质激素和热休克蛋白70均呈双峰曲线模式变化,且变化趋势相似,而电针治疗能明显降低促肾上腺皮质激素和热休克蛋白70的峰值水平。结果说明电针可能通过抑制机体过度应激、减轻炎性损伤过程,促进神经元的修复,发挥其对缺血性脑卒中的保护和治疗作用。

ORCID:0000-0001-8576-3311(Ya Tu)

关键词: 神经再生, 电针, 脑缺血再灌注, 炎症, 促肾上腺皮质激素, 热休克蛋白70, 百会, 足三里

Abstract:

Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are released by damaged cells, which can lead to systemic stress reactions. Protecting the brain through repair begins with the stress-injury-repair signaling chain. This study aimed to verify whether acupuncture acts through this chain to facilitate effective treatment of ischemic stroke. Rat models of cerebral ischemia/reperfusion injury were established by Zea Longa’s method, and injury sites were identified by assessing neurological function, 2,3,5-triphenyltetrazolium chloride staining, and hematoxylin-eosin staining. Electroacupuncture at acupoints Baihui (DU20) and Zusanli (ST36) was performed in the model rats with dilatational waves, delivered for 20 minutes a day at 2–100 Hz and an amplitude of 2 mA. We analyzed the blood serum from the rats and found that inflammatory cytokines affected the levels of adrenotrophin and heat shock protein 70, each of which followed a similar bimodal curve. Specifically, electroacupuncture lowered the peak levels of adrenocorticotrophic hormone and heat shock protein 70. Thus, electroacupuncture was able to inhibit excessive stress, reduce inflammation, and promote the repair of neurons, which facilitated healing of ischemic stroke.

Key words: nerve regeneration, electroacupuncture, cerebral ischemia/reperfusion, inflammation, adrenocorticotrophic hormone, heat shock protein 70, Baihui (DU20), Zusanli (ST36), neural regeneration