中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (7): 1159-1165.doi: 10.4103/1673-5374.211197

• 原著:退行性病与再生 • 上一篇    下一篇

PET观察长期针刺足三里穴对阿尔茨海默病大鼠脑区的多靶点调控

  

  • 收稿日期:2017-05-24 出版日期:2017-07-15 发布日期:2017-07-15
  • 基金资助:

    973项目（2006CB504505, 2012CB518504）; 国家自然科学基金(90709027)南方医科大学大学生创业训练项目（201512121165）; 广东医科大学博士基金（2XB13058）.

Long-term acupuncture treatment has a multi-targeting regulation on multiple brain regions in rats with Alzheimer’s disease: a positron emission tomography study

Yang-jia Lu1, 2, Xiao-wen Cai1, Gui-feng Zhang3, Yong Huang1, Chun-zhi Tang4, Bao-ci Shan5, Shao-yang Cui4, 6, Jun-qi Chen7, Shan-shan Qu1, Zheng Zhong1, Xin-sheng Lai4, Genevieve Zara Steiner8   

  1. 1 School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China; 2 Traditional Chinese Medicine of the Second Clinical School, Guangdong Medical College, Dongguan, Guangdong Province, China; 3 Zhaoqing Medical College, Zhaoqing, Guangdong Province, China; 4 Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China; 5 Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China; 6 Shenzhen Hospital of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, China; 7 Huarui Hospital of Southern Medical University, Guangzhou, Guangdong Province, China; 8 National Institute of Complementary Medicine, Western Sydney University, Penrith NSW, Australia
  • Received:2017-05-24 Online:2017-07-15 Published:2017-07-15
  • Contact: Yong Huang, Ph.D. or Chun-zhi Tang, Ph.D., nanfanglihuang@163.com or jordan664@163.com.
  • Supported by:

    This study was supported by the National Basic Research Program of China (973 Program), No. 2006CB504505, 2012CB518504; the National Natural Science Foundation of China, No. 90709027; the Student’s Platform for Innovation and Entrepreneurship Training Program
    of Southern Medical University of China, No. 201512121165; the Doctoral Foundation of Guangdong Medical University of China, No. 2XB13058.

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摘要：

分析针刺治疗阿尔茨海默病的即刻效应，即在针刺状态下脑区的激活情况已有报道，但有关长期针刺对阿尔茨海默病脑区的多靶点调控报道较少。为此，实验建立了阿尔茨海默病大鼠模型，给予针刺右侧足三里穴(ST36)和右侧非穴(足三里旁开1.5 mm)，1次/d，每次持续30 min，针刺30 d，对接受针刺大鼠头部PET扫描，像素统计分析软件SPM2分析图像显示，针刺足三里穴30 d后，大鼠左侧海马、左侧额眶部皮质、左侧下边缘皮质、左侧嗅皮质区、左侧小脑和左侧脑桥区被激活，而非穴组的结果与足三里穴组基本类似。相比于非穴，针刺足三里更能显著激活小脑和脑桥区。数据显示，长期针刺足三里对于阿尔茨海默病大鼠脑区具有多靶点调控作用。

ORCID:0000-0003-2652-8586(Yong Huang); 0000-0003-3127-578X(Chun-zhi Tang)

关键词: 神经再生, 神经退行性变, 针灸, 足三里, 阿尔茨海默病, 长期治疗, 正电子发射型断层显像技术, 大鼠, 机制, 靶点作用, 代偿作用, 多靶点调控作用

Abstract:

The acute effect of acupuncture on Alzheimer’s disease, i.e., on brain activation during treatment, has been reported. However, the effect of long-term acupuncture on brain activation in Alzheimer’s disease is unclear. Therefore, in this study, we performed long-term needling at Zusanli (ST36) or a sham point (1.5 mm lateral to ST36) in a rat Alzheimer’s disease model, for 30 minutes, once per day, for 30 days. The rats underwent 18F-fluorodeoxyglucose positron emission tomography scanning. Positron emission tomography images were processed with SPM2. The brain areas activated after needling at ST36 included the left hippocampus, the left orbital cortex, the left infralimbic cortex, the left olfactory cortex, the left cerebellum and the left pons. In the sham-point group, the activated regions were similar to those in the ST36 group. However, the ST36 group showed greater activation in the cerebellum and pons than the sham-point group. These findings suggest that long-term acupuncture treatment has targeted regulatory effects on multiple brain regions in rats with Alzheimer’s disease.

Key words: nerve regeneration, neurodegeneration, acupuncture;, Zusanli (ST36), Alzheimer’s disease, long-term treatment, positron emission tomography, rat, mechanism, targeting effect, compensation, multi-target regulation, neural regeneration