中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (11): 1953-1960.doi: 10.4103/1673-5374.238718

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

快速显微磁共振成像神经扫描：用于周围神经束型结构三维重建

  

  • 收稿日期:2018-06-22 出版日期:2018-11-15 发布日期:2018-11-15
  • 基金资助:

    国家重点研究与发展计划(31670986)；广东省科技项目（2014B020227001，2017A050501017）；中国广州科技项目（201807010082，201704030041)

A rapid micro-magnetic resonance imaging scanning for three-dimensional reconstruction of peripheral nerve fascicles

Zhi Yao1, 2, Li-Wei Yan1, 2, Tao Wang1, 2, Shuai Qiu1, 2, Tao Lin1, 2, Fu-Lin He1, 2, Ru-Heng Yuan1, 2, Xiao-Lin Liu1, 2, 3, Jian Qi1, 2, 3, Qing-Tang Zhu1, 2, 3   

  1. 1 Department of Microsurgery and Orthopedic Trauma, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
    2 Center for Peripheral Nerve Tissue Engineering and Technology Research, Guangzhou, Guangdong Province, China
    3 Guangdong Province Engineering Laboratory for Soft Tissue Biofabrication, Guangzhou, Guangdong Province, China
  • Received:2018-06-22 Online:2018-11-15 Published:2018-11-15
  • Contact: Qing-Tang Zhu, MD, PhD or Jian Qi, MD, PhD,zhuqingt@mail.sysu.edu.cn or speedsnow@126.com.
  • Supported by:

    This study was supported by grants from the National Key Research and Development Plan of China, No. 31670986 (to QTZ); the Science and Technology Project of Guangdong Province of China, No. 2014B020227001, 2017A050501017 (to QTZ); the Science and Technology Project of Guangzhou of China, No. 201807010082 (to QTZ), 201704030041 (to JQ).

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摘要：

研究周围神经结构的主要方法是结合显微解剖、组织化学染色及连续切片等方式进行三维重建，然而该方法存在图像配准欠佳的缺点。新兴医学影像技术Micro-MRI成像在脑组织、肝组织软组织扫描有较多应用，然而尚未有研究报道Micro-MRI在获得周围神经束型结构图像。故实验拟观察应用1T Micro-MRI获得周围神经内部图像的可行性。选择3例成年截肢患者的下肢坐骨神经为研究样本，将神经短段随机分到4组不同的扫描环境中：钆剂环境下T1和T2序列扫描，蒸馏水环境下T1和T2序列扫描，应用标准化参数对4组标本进行扫描。由3位临床医生通过标准化的量表对图像质量进行了评估。为了分析二维图像的变形情况，将Micro-MRI扫描后的神经标本进行组织学切片苏木精-伊红染色，统计神经直径和总面积数据。试验结果显示，钆剂环境下T1序列扫描的Micro-MRI图像效果最佳，连续扫描图像可用周围神经神经束结构的三维重建。与组织学图像相比，Micro-MRI图像具有良好的稳定性和较小的变形率。上述数据结果说明，钆剂环境下T1序列扫描的Micro-MRI，为周围神经束的三维重建提供了一种可行、快速的方法，可清晰显示周围神经内部结构。

orcid:0000-0001-6256-1602(Qing-Tang Zhu)
        0000-0003-4993-1335(Jian Qi)

关键词: 周围神经, 束状三维空间重建, 束状地形图, 显微MRI, 快速获取图像, 造影剂, Mannerist液, 组织学技术, 变形分析, 周围神经损伤, 神经再生

Abstract:

The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging (Micro-MRI), an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by 1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions (Mannerist Solution/GD-DTPA contrast agent, distilled water) were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent (Mannerist Solution/GD-DTPA) and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.

Key words: nerve regeneration, peripheral nerve, fascicular three-dimensional reconstruction, fascicular topography, micro-magnetic resonance imaging, rapid acquired images, contrast agent, Mannerist Solution, histological techniques, deformation analysis, peripheral nerve injury, neural regeneration