中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (6): 1085-1090.doi: 10.4103/1673-5374.250630

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

脂肪干细胞分化的神经样细胞可治疗大鼠海绵体神经损伤

  

  • 出版日期:2019-06-15 发布日期:2019-06-15
  • 基金资助:

    武汉中心医院基础研究基金项目（YB16A01），湖北省自然科学基金项目（2017CFB176）

Neural-like cells from adipose-derived stem cells for cavernous nerve injury in rats

Cheng-Cheng Ying 1 , Mei Yang 2 , Yong Wang 1 , Yong-Lian Guo 1 , Wan-Li Hu 3 , Xin-Min Zheng 3   

  1. 1 Department of Urology, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
    2 Department of Endocrinology, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan, Hubei Province, China
    3 Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, China
  • Online:2019-06-15 Published:2019-06-15
  • Contact: Yong-Lian Guo, PhD, 357039334@qq.com.
  • Supported by:

    This study was supported by the Fundamental Research Funds for the Central Hospital of Wuhan of China, No. YB16A01 (to CCY); and the Natural Science Foundation of Hubei Province of China, No. 2017CFB176 (to CCY).

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摘要：

当海绵体神经被压迫/切断且丧失神经功能时，虽然剩余神经组织可再生并部分恢复勃起功能，但这些神经组织再生能力有限往往达不到临床需求。而研究显示脂肪干细胞易于获得和培养，可分化为神经细胞，且增殖速度易于控制，可能帮助损伤海绵体神经的恢复功能。实验设计以夹伤双侧海绵体神经建立大鼠海绵体神经损伤模型，然后在海绵体内立即注射100万个由脂肪干细胞分化的神经样细胞。以刺激海绵体评估勃起功能，以甲苯胺蓝染色检测海绵体背神经中有髓鞘轴突的数量，以免疫组化染色检测阴茎背神经中的神经元型一氧化氮合酶阳性纤维的面积，以Masson染色分析阴茎组织中平滑肌/胶原比率。结果显示，海绵体神经损伤大鼠经细胞移植治疗后，最大海绵体内压水平以及最大海绵体内压与平均动脉压比值上升，海绵体背神经中有髓鞘轴突和神经元型一氧化氮合酶阳性神经纤维的数量增加，平滑肌/胶原比率上升。表明脂肪干细胞诱导分化的神经样细胞可有效减轻海绵体神经损伤，改善其勃起功能。

orcid: 0000-0002-9899-3869 (Yong-Lian Guo)

关键词: 脂肪源性神经干细胞, 海绵体, 海绵体神经, 勃起功能障碍, 前列腺癌根治, 神经元, 细胞分化, 神经再生

Abstract:

Although the remaining nerve tissue can regenerate and partly restore erectile function when the cavernous nerve is compressed/severed and function lost, the limited regenerative ability of these nerve tissues often fails to meet clinical needs. Adipose-derived stem cells are easy to obtain and culture, and can differentiate into neural cells. Their proliferation rate is easy to control and they may be used to help restore injured cavernous nerve function. Sprague-Dawley male rats (n = 45) were equally randomized into three groups: fifteen rats as a sham-op¬erated group, fifteen rats as a bilateral nerve crush (BINC) group (with no further intervention), fifteen rats as a BINC with intracavernous injection of one million neural-like cells from adipose-derived stem cells (NAS) (BINC + NAS) group. After 4 weeks, erectile function was assessed by stimulating the cavernous body. The number of myelinated axons in the dorsal cavernous nerve was determined by toluidine blue staining. The area of neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve was measured by immunohistochemical staining. Masson staining was used to analyze the ratio of smooth muscle to collagen in penile tissue. The results demonstrate that maxi¬mal intracavernous pressure, the ratio of maximal intracavernous pressure to mean arterial pressure, the numbers of myelinated axons and neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve, and the ratio of smooth muscle to collagen could be increased after cell transplantation. These findings indicate that neural-like cells from adipose-derived stem cells can effectively alleviate cavernous nerve injury and improve erectile function.

Key words: nerve regeneration, adipose-derived neural stem cells, corpus cavernosum, cavernous nerve, erectile dysfunction, radical prostatectomy, neurons, cell differentiation, neural regeneration