中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (7): 1230-1236.doi: 10.4103/1673-5374.251330

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

早期平板运动提高脑缺血再灌注后巨噬细胞移动抑制因子的表达

  

  • 出版日期:2019-07-15 发布日期:2019-07-15
  • 基金资助:

    该研究得到了韩国教育部国家研究基金会（NRF）的基础科学研究计划的支持

Early treadmill exercise increases macrophage migration inhibitory factor expression after cerebral ischemia/reperfusion

Min Cheol Chang 1 , Chae Ri Park 2 , Seung Hwa Rhie 3 , Woo Hyun Shim 2, 4 , Dae Yul Kim 3   

  1. 1 Department of Rehabilitation Medicine, College of Medicine, Yeungnam University, Daegu, Republic of Korea
    2 Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
    3 Department of Rehabilitation Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
    4 Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
  • Online:2019-07-15 Published:2019-07-15
  • Contact: Dae Yul Kim, MD, dykimsmart@gmail.com.
  • Supported by:

    This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, No. 2016R1A2B4012772 (to DYK).

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摘要：

研究显示，巨噬细胞移动抑制因子（MIF）在缺血事件期间具有保护功能；然而，这些研究中主要涉及肠缺血和心肌梗死，其在缺血性脑卒中的神经保护中的作用则少见报道。为观察早期平板运动对缺血性卒中恢复的影响，并确定这些作用是否与缺血区巨噬细胞移动抑制因子和脑源性神经营养因子（BDNF）的表达水平相关。实验将40只雄性SD大鼠随机分配至缺血+运动组、缺血+久坐组、假手术+运动组、假手术+久坐组。运动组大鼠于大脑中动脉阻塞后48h,在跑步机上跑步30min，连续5d。相较于缺血+久坐组，缺血+运动组线悬挂试验测试的悬挂时间更长，缺血半暗带中MRI检测的相对各向异性分数（rFA）更高，缺血半暗带中巨噬细胞移动抑制因子和脑源性神经营养因子的表达水平上调。增加的巨噬细胞移动抑制因子和脑源性神经营养因子水平与缺血半暗带的相对各向异性分数变化呈明显正相关。两个假手术组之间的缺血半暗带中巨噬细胞移动抑制因子和脑源性神经营养因子的水平相近。研究表明，早期运动（大脑中动脉阻塞后48h开始）可以改善脑缺血再灌注后的神经功能和损伤神经组织的恢复；缺血半暗带中巨噬细胞移动抑制因子和脑源性神经营养因子水平的增加可能是神经功能恢复的机制之一。

orcid: 0000-0003-1275-1736 (Dae Yul Kim)

关键词: 缺血性脑卒中, 早期运动, 巨噬细胞移动抑制因子, 脑源性神经营养因子, 运动恢复, 神经再生

Abstract:

The neuroprotective function of macrophage migration inhibitory factor (MIF) in ischemic stroke was rarely evaluated. This study aimed to investigate the effects of early treadmill exercise on recovery from ischemic stroke and to determine whether these effects are associated with the expression levels of MIF and brain-derived neurotrophic factor (BDNF) in the ischemic area. A total of 40 male Sprague-Dawley rats were randomly assigned to the ischemia and exercise group [middle cerebral artery occlusion (MCAO)-Ex, n = 10), ischemia and sedentary group (MCAO-St, n = 10), sham-surgery and exercise group (Sham-Ex, n = 10), or sham-surgery and sedentary group (Sham-St, n = 10). The MCAO-Ex and MCAO-St groups were subjected to MCAO for 60 minutes, whereas the Sham-Ex and Sham-St groups were subjected to an identical operation without MCAO. Rats in the MCAO-Ex and Sham-Ex groups then ran on a treadmill for 30 minutes once a day for 5 consecutive days. After reperfusion, the hanging time tested by the wire hang test was longer and the relative fractional anisotropy (rFA) determined by MRI was higher in the peri-infarct region of the MCAO-Ex group compared with the MCAO-St group. The expression levels of MIF and BDNF in the peri-infarct region were upregulated in the MCAO-Ex group. Increased MIF and BDNF levels were positively correlated with rFA changes in the peri-infarct region. There was no significant difference in the levels of MIF and BDNF in the peri-infarct region between the Sham-Ex and Sham-St groups. Our study demonstrated that early exercise (initiated 48 hours after the MCAO) could improve motor and neuronal recovery after ischemic stroke. Furthermore, the increased levels of MIF and BDNF in the peri-infarct region (penumbra) may be one of the mechanisms of enhanced neurological function recovery.

Key words: ischemic stroke, early exercise, macrophage migration inhibitory factor, brain-derived neurotrophic
factor, motor recovery, neural regeneration