中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (10): 1955-1961.doi: 10.4103/1673-5374.280324

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

锂剂具有促进许旺细胞增殖但抑制其迁移的能力

  

  • 出版日期:2020-10-15 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金（81970820）

Lithium promotes proliferation and suppresses migration of Schwann cells

Xiao-Kun Gu1, 2, Xin-Rui Li3, Mei-Ling Lu3, Hui Xu1   

  1. 1 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
    2 Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
    3 State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu Province, China
  • Online:2020-10-15 Published:2020-08-19
  • Contact: Hui Xu, MD, huixu82@126.com or huixu@ntu.edu.cn; Mei-Ling Lu, lumeiling@cpu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81970820 (to HX).

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摘要：

许旺细胞的增殖、迁移和其髓鞘化作用对于受损周围神经系统的再生至关重要。以往研究显示锂剂可促进许旺细胞的髓鞘化作用而改善周围神经再生。然而，锂剂是否会调控许旺细胞的其他表型，如增殖和迁移尚不清楚。实验从正常大鼠坐骨神经中分离和原代培养了许旺细胞。为了解锂剂对许旺细胞表型的影响，以不同浓度的氯化锂(0, 5, 10, 15, 30 mM)处理许旺细胞24 h，并通过CCK8、EdU、划痕和侵袭实验等手段验检测锂对许旺细胞增殖和迁移的影响。(1)CCK8和EdU增殖实验显示：5, 10, 15, 30 mM的氯化锂显著地提高了许旺细胞的活力和增殖率；(2)划痕和侵袭实验显示：10, 15, 30 mM的氯化锂可抑制许旺细胞的迁移能力；(3)氯化锂对许旺细胞增殖和迁移的影响：总体上呈明显的剂量依赖效应；(4)上述结果提示：锂剂具有促进许旺细胞增殖但抑制其迁移的能力，这一结论有可能提供促进周围神经修复与再生治疗策略的设计思路。

orcid: 0000-0003-0782-4160 (Hui Xu) 

         0000-0001-8738-987X (Mei-Ling Lu)

关键词: CCK8, 细胞活力, EdU, 锂, 细胞迁移, 周围神经, 细胞增殖, 再生, 许旺细胞, 划痕实验

Abstract: Schwann cell proliferation, migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system injury. Lithium promotes remyelination by Schwann cells and improves peripheral nerve regeneration. However, whether lithium modulates other phenotypes of Schwann cells, especially their proliferation and migration remains elusive. In the current study, primary Schwann cells from rat sciatic nerve stumps were cultured and exposed to 0, 5, 10, 15, or 30 mM lithium chloride (LiCl) for 24 hours. The effects of LiCl on Schwann cell proliferation and migration were examined using the Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, Transwell and wound healing assays. Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine assays showed that 5, 10, 15, and 30 mM LiCl significantly increased the viability and proliferation rate of Schwann cells. Transwell-based migration assays and wound healing assays showed that 10, 15, and 30 mM LiCl suppressed the migratory ability of Schwann cells. Furthermore, the effects of LiCl on the proliferation and migration phenotypes of Schwann cells were mostly dose-dependent. These data indicate that lithium treatment significantly promotes the proliferation and inhibits the migratory ability of Schwann cells. This conclusion will inform strategies to promote the repair and regeneration of peripheral nerves. All of the animal experiments in this study were ethically approved by the Administration Committee of Experimental Animal Center of Nantong University, China (approval No. 20170320-017) on March 2, 2017.

Key words: 5-ethynyl-2′-deoxyuridine, Cell Counting Kit-8, cell viability, lithium, migration, peripheral nerve, proliferation, regeneration, Schwann cell, wound healing assay