中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (11): 2284-2292.doi: 10.4103/1673-5374.310698

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

负载神经干细胞的多通道胶原支架修复脊髓损伤

  

  • 出版日期:2021-11-15 发布日期:2021-04-13
  • 基金资助:

    中国国家重点研究开发计划项目（2017YFA04304）；国家自然科学基金项目（81571213，81800583，81601539）；南京市医学技术发展基金项目（QRX17006，QRX17057）；医学科技发展基金会-南京卫委-南京市医学科技创新平台（ZDX16005）；重庆渝中区科委项目（20140112）

A multi-channel collagen scaffold loaded with neural stem cells for the repair of spinal cord injury

Shuo Liu, Yuan-Yuan Xie, Liu-Di Wang, Chen-Xu Tai, Dong Chen, Dan Mu, Yan-Yan Cui3, Bin Wang1, *#br#   

  1. 1Clinical Stem Cell Center, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China; 2Department of Radiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China; 3Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing, China
  • Online:2021-11-15 Published:2021-04-13
  • Contact: Bin Wang, PhD, wangbin022800@126.com.
  • Supported by:
    The study was supported by the National Key Research and Development Program of China, No. 2017YFA0104304 (to NG); the National Natural Science Foundation of China, Nos. 81571213 (to BW), 81800583 (to YYX), 81601539 (to DM); the Nanjing Medical Science and Technique Development Foundation of China, Nos. QRX17006 (to BW), QRX17057 (to DM); the Key Project Supported by Medical Science and Technology Development Foundation, Nanjing Department of Health and the Nanjing Medical Science and Innovation Platform of China, No. ZDX16005 (to BW); and Chongqing Yuzhong District Science and Technology Commission Project of China, No. 20140112 (to YYC).

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摘要：

由于胶原蛋白支架具有三维空间多孔结构，以便为细胞的生长和增殖提供足够的空间，以及容纳足够的营养和氧气，并释放代谢产物，实验因此设计了一种新型具有轴向排列的多通道胶原支架。（1）体外评估这种支架对胎鼠海马神经干细胞的生物相容性，发现支架可增强细胞的活性，有助于细胞延伸，对细胞分化无明显影响；（2）将负载神经干细胞的支架置入T8完全横断大鼠模型中，发现其可显著改善大鼠的后肢运动功能，并促进神经再生；同时其生物降解性良好，在体内5周内可完全降解；且直肠温度、C-反应蛋白以及CD68染色等证实植入该支架的脊髓损伤大鼠未发生明显的炎症反应；（3）提示这种新型胶原支架是神经干细胞移植治疗的良好载体，有助于修复脊髓损伤。实验于2019年6月15日经南京大学医学院附属鼓楼医院研究伦理委员会批准（批准号2019AE02005）。

https://orcid.org/0000-0003-3981-8849 (Bin Wang)

关键词:

脊髓损伤, 胶原, 生物材料, 轴向排列孔道, 神经干细胞, 组织工程, 支架, 完全横断损伤, 细胞移植, 炎症, 再生

Abstract: Collagen scaffolds possess a three-dimensional porous structure that provides sufficient space for cell growth and proliferation, the passage of nutrients and oxygen, and the discharge of metabolites. In this study, a porous collagen scaffold with axially-aligned luminal conduits was prepared. In vitro biocompatibility analysis of the collagen scaffold revealed that it enhances the activity of neural stem cells and promotes cell extension, without affecting cell differentiation. The collagen scaffold loaded with neural stem cells improved the hindlimb motor function in the rat model of T8 complete transection and promoted nerve regeneration. The collagen scaffold was completely degraded in vivo within 5 weeks of implantation, exhibiting good biodegradability. Rectal temperature, C-reactive protein expression and CD68 staining demonstrated that rats with spinal cord injury that underwent implantation of the collagen scaffold had no notable inflammatory reaction. These findings suggest that this novel collagen scaffold is a good carrier for neural stem cell transplantation, thereby enhancing spinal cord repair following injury. This study was approved by the Animal Ethics Committee of Nanjing Drum Tower Hospital (the Affiliated Hospital of Nanjing University Medical School), China (approval No. 2019AE02005) on June 15, 2019.

Key words: axially-aligned luminal conduits, biomaterial, cell transplantation, collagen, complete transection, inflammation, neural stem cell, regeneration scaffold, spinal cord injury, tissue engineering

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