中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (3): 594-600.doi: 10.4103/1673-5374.320992

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

刺激耳迷走神经保护脑缺血再灌注损伤的作用机制

  

  • 出版日期:2022-03-15 发布日期:2021-10-15

The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury

Jing-Jun Zhao1, 2, 3, Zheng-Hui Wang4, Ying-Jie Zhang5, Wen-Jing Wang1, 2, Ai-Fang Cheng5, Pei-Jing Rong6, Chun-Lei Shan1, 2, 3, *#br#   

  1. 1Center of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 3Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, China; 4Center of Rehabilitation Medicine, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan Province, China; 5Shanghai Research Institute of Acupuncture and Meridian, Shanghai, China; 6Institute of Acupuncture and Moxibustion, Chinese Academy of Chinese Medical Sciences, Beijing, China
  • Online:2022-03-15 Published:2021-10-15
  • Contact: Chun-Lei Shan, PhD, MD, shanclhappy@163.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81704163 (to JJZ), 81874035 (to CLS); the National Key R&D Program of China, No. 2018YFC2001600 (to CLS); the Shanghai Health Commission Accelerated the Development of Traditional Chinese Medicine Three-Year Action Plan Project of China, No. ZY(2018-2020)-CCCX-2001-06/2004-05 (to CLS); and the Program of Shanghai Academic Research Leader of China, No. 19XD1403600 (to CLS).

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摘要：

既往研究表明，迷走神经刺激可改善患者的运动功能，而耳迷走神经作为迷走神经的惟一浅表分支，可能有与迷走神经刺激类似的效果，但其具体机制尚不明确。（1）实验采用改良的Longa线栓法建立了脑缺血再灌注损伤大鼠模型，24h后于耳部进行7d迷走神经刺激；（2）见刺激耳迷走神经促进了缺血性脑卒中大鼠乙酰胆碱的分泌，抑制了缺血半暗带及运动皮质中白细胞介素1β、白细胞介素6和肿瘤坏死因子α的分泌以及连接蛋白43的磷酸化，并促进运动功能的恢复；（3）提示刺激耳迷走神经通过影响乙酰胆碱和炎症因子的分泌以及连接蛋白43的磷酸化，对脑缺血再灌注损伤后大鼠的运动功能的恢复发挥了作用。实验于2019年11月8日经上海中医药大学动物伦理委员会批准（批准号PZSHUTCM191108014）。

https://orcid.org/0000-0001-9593-6722 (Jing-Jun Zhao); https://orcid.org/0000-0002-2742-1281 (Chun-Lei Shan)

关键词: 缺血再灌注损伤, 脑卒中, 耳迷走神经刺激, 间隙连接, 神经功能, 炎性因子, 连接蛋白43, 神经保护

Abstract: Previous studies have shown that vagus nerve stimulation can improve patients’ locomotor function. The stimulation of the auricular vagus nerve, which is the only superficial branch of the vagus nerve, may have similar effects to vagus nerve stimulation. However, the precise mechanisms remain poorly understood. In this study, rat models of cerebral ischemia/reperfusion injury were established by modified Longa ligation. Twenty-four hours later, 7-day auricular vagus nerve stimulation was performed. The results showed that auricular vagus nerve stimulation promoted the secretion of acetylcholine, inhibited the secretion of interleukin-1β, interleukin-6, and tumor necrosis factor-α, and reduced connexin 43 phosphorylation in the ischemic penumbra and motor cortex, promoting locomotor function recovery in rats with cerebral ischemia/reperfusion injury. These findings suggested that auricular vagus nerve stimulation promotes the recovery of locomotor function in rats with cerebral ischemia/reperfusion injury by altering the secretion of acetylcholine and inflammatory factors and the phosphorylation of connexin 43. This study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine on November 8, 2019 (approval No. PZSHUTCM191108014).

Key words: auricular vagus nerve stimulation, connexin 43, gap junction, inflammatory factor, ischemia/reperfusion injury, neurological function, neuroprotection, stroke

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