中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (7): 1545-1555.doi: 10.4103/1673-5374.330617

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针可改善脑缺血再灌注损伤后的运动和神经功能

  

  • 出版日期:2022-07-15 发布日期:2022-01-17
  • 基金资助:
    国家重点研发计划项目(2018YFC2001600);国家自然科学基金项目(81902301);上海中医药大学预算项目(2019LK024);上海市卫健委智慧医疗项目(2018ZHYL0216);上海申康医院发展中心临床科技创新项目(SHDC12018126);上海市卫健委加快推进中医药发展3年行动计划项目(ZY(2018-2020)-CCCX-2001-06,ZY(2018-2020)-CCCX-2004-05)

Electroacupuncture treatment improves motor function and neurological outcomes after cerebral ischemia/reperfusion injury

Si-Si Li1, Xu-Yun Hua2, Mou-Xiong Zheng2, Jia-Jia Wu3, Zhen-Zhen Ma1, Xiang-Xin Xing1, Jie Ma1, Chun-Lei Shan1, 3, 4, Jian-Guang Xu1, 4, *   

  1. 1School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Department of Traumatology and Orthopedics, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 3Center of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 4Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, China
  • Online:2022-07-15 Published:2022-01-17
  • Contact: Jian-Guang Xu, PhD, xjg@shutcm.edu.cn.
  • Supported by:
    This work was supported by the National Key R&D Program of China, No. 2018YFC2001600 (to JGX), the National Natural Science Foundation of China, No. 81902301 (to JJW), Budgetary Project of Shanghai University of Traditional Chinese Medicine of China, No. 2019LK024 (to JJW), Intelligent Medical Program of Shanghai (Municipal) Health Commission of China, No. 2018ZHYL0216 (to CLS), Clinical Science and Technology Innovation Project of Shanghai Shen Kang Hospital Development Center of China, No. SHDC12018126 (to CLS), Accelerated the Development of Traditional Chinese Medicine Three-Year Action Plan Project (of Shanghai Health Commission) of China, Nos. ZY(2018-2020)-CCCX-2001-06 (to JGX and CLS) and ZY(2018-2020)-CCCX-2004-05 (to JGX and CLS).

摘要:

电针已被广泛用于促进脑卒中的功能恢复,但其在脑卒中后康复中的作用和调节机制仍知之甚少。实验对大脑中动脉闭塞再灌注大鼠进行足三里(ST36)和曲池(LI11)进行电针刺激后发现,电针能有效促进大脑中动脉闭塞再灌注大鼠脑组织缺血半暗带中脑源性神经营养因子及其受体TrkB以及突触相关蛋白synapsin-1、突触后致密蛋白95和微管相关蛋白2的表达,显著促进运动功能恢复和神经可塑性,同时还能有效减少缺血半暗带组织中髓鞘相关抑制因子Nogo-A及NgR的表达。而TrkB抑制剂ANA-12则能减弱电针的治疗效果。综上表明,电针改善大脑中动脉闭塞再灌注后神经功能可能是通过调节脑源性神经营养因子/TrkB信号通路实现的。实验于2020年1月10日经上海中医药大学动物伦理委员会批准(批准号PZSHUTCM200110002)。

https://orcid.org/0000-0003-0601-3403 (Jian-Guang Xu)

关键词: 电针, 缺血再灌注, 运动功能, 神经学预后, 脑源性神经营养因子, TrkB, 神经突生长抑制剂A, Nogo受体, 突触, 树突

Abstract: Electroacupuncture (EA) has been widely used for functional restoration after stroke. However, its role in post-stroke rehabilitation and the associated regulatory mechanisms remain poorly understood. In this study, we applied EA to the Zusanli (ST36) and Quchi (LI11) acupoints in rats with middle cerebral artery occlusion and reperfusion. We found that EA effectively increased the expression of brain-derived neurotrophic factor and its receptor tyrosine kinase B, synapsin-1, postsynaptic dense protein 95, and microtubule-associated protein 2 in the ischemic penumbra of rats with middle cerebral artery occlusion and reperfusion. Moreover, EA greatly reduced the expression of myelin-related inhibitors Nogo-A and NgR in the ischemic penumbra. Tyrosine kinase B inhibitor ANA-12 weakened the therapeutic effects of EA. These findings suggest that EA can improve neurological function after middle cerebral artery occlusion and reperfusion, possibly through regulating the activity of the brain-derived neurotrophic factor/tyrosine kinase B signal pathway. All procedures and experiments were approved by the Animal Research Committee of Shanghai University of Traditional Chinese Medicine, China (approval No. PZSHUTCM200110002) on January 10, 2020.

Key words: brain-derived neurotrophic factor, dendritic, electroacupuncture, ischemia/reperfusion, motor function, neurite outgrowth inhibitor-A, neurological outcomes, Nogo receptor, synapse, tyrosine kinase B