中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (6): 1336-1343.doi: 10.4103/1673-5374.385844

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

运动联合褪黑素可改善缺血性脑卒中大鼠的睡眠及运动障碍

  

  • 出版日期:2024-06-15 发布日期:2023-11-18
  • 基金资助:
    中国康复研究中心项目(2021zx-03),康复大学与中国康复研究院博士生联合培养专项基金(2020 kfdx-008)

Exercise-with-melatonin therapy improves sleep disorder and motor dysfunction in a rat model of ischemic stroke

Haitao Zhao1, 2, 3, 4, Tong Zhang1, 2, 3, 4, *, Haojie Zhang1, 2, 4, Yunlei Wang1, 2, 4, Lingna Cheng1, 2, 4   

  1. 1School of Rehabilitation Medicine, Capital Medical University, Beijing, China; 2Department of Neurological Rehabilitation, Beijing Bo’ai Hospital, China Rehabilitation Research Center, Beijing, China; 3School of Rehabilitation Medicine, University of Health and Rehabilitation Sciences, Qingdao, Shandong Province, China; 4Laboratory of Brain Injury Repair and Rehabilitation, China Rehabilitation Science Institute, Beijing, China
  • Online:2024-06-15 Published:2023-11-18
  • Contact: Tong Zhang, PhD, tommzhang@163.com.
  • Supported by:
    This study was supported by China Rehabilitation Research Center, No. 2021zx-03 and the Special Fund for Joint Training of Doctoral Students between the University of Health and Rehabilitation Sciences and China Rehabilitation Research Center, No. 2020 kfdx-008 (both to TZ).

摘要:

运动联合褪黑素治疗对脊髓损伤和阿尔茨海默病有互补和协同改善作用,但其对脑卒中的影响还知之甚少。实验通过阻塞雄性SD大鼠大脑中动脉60min建立了缺血性脑卒中模型,然后连续7d给予运动联合褪黑素干预。结果可见,运动联合睡眠可显著延长脑卒中大鼠的睡眠时间,并增加delta功率值并恢复delta功率节律。此外,运动联合睡眠还能有效改善脑卒中大鼠的协调性、耐力和握力以及学习记忆能力。同时脑卒中大鼠运动联合睡眠治疗后,其海马CA1区神经元活性和突触后密度厚度明显增加,细胞外谷氨酸含量降低,而谷氨酸受体2的表达增加。且运动联合褪黑素治疗比单一褪黑素或运动治疗更为有效。这提示,运动联合睡眠联合治疗可改善脑卒中后睡眠障碍以及运动功能,其作用是通过诱导谷氨酸2型受体蛋白表达的增加以及调节海马CA1区突触可塑性实现的。

https://orcid.org/0000-0003-0898-1766 (Tong Zhang); https://orcid.org/0000-0003-3725-4787 (Haitao Zhao)

关键词: 缺血性脑卒中, 睡眠障碍, 褪黑素, 运动, 谷氨酸受体2, 海马, 突触后密度, 突触可塑性, 学习, 记忆

Abstract: Exercise-with-melatonin therapy has complementary and synergistic effects on spinal cord injury and Alzheimer’s disease, but its effect on stroke is still poorly understood. In this study, we established a rat model of ischemic stroke by occluding the middle cerebral artery for 60 minutes. We treated the rats with exercise and melatonin therapy for 7 consecutive days. Results showed that exercise-with-melatonin therapy significantly prolonged sleep duration in the model rats, increased delta power values, and regularized delta power rhythm. Additionally, exercise-with-melatonin therapy improved coordination, endurance, and grip strength, as well as learning and memory abilities. At the same time, it led to higher hippocampal CA1 neuron activity and postsynaptic density thickness and lower expression of glutamate receptor 2 than did exercise or melatonin therapy alone. These findings suggest that exercise-with-melatonin therapy can alleviate sleep disorder and motor dysfunction by increasing glutamate receptor 2 protein expression and regulating hippocampal CA1 synaptic plasticity.

Key words: exercise, glutamate receptor 2, hippocampus, ischemic stroke, learning, melatonin, memory, sleep disorder, synaptic plasticity