中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (24): 2266-2274.doi: 10.3969/j.issn.1673-5374.2013.24.006

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

纳米纤维神经导管修复30mm长段坐骨神经缺损

  

  • 收稿日期:2013-05-02 修回日期:2013-06-08 出版日期:2013-08-25 发布日期:2013-08-25

Nanofibrous nerve conduits for repair of 30-mm-long sciatic nerve defects

Esmaeil Biazar1, Saeed Heidari Keshel2, 3, Majid Pouya4, Hadi Rad4, Melody Omrani Nava4, Mohammad Azarbakhsh5, Shirin Hooshmand5   

  1. 1 Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
    2 Student Research Committee, Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    3 Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    4 Department of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
    5 Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Iran
  • Received:2013-05-02 Revised:2013-06-08 Online:2013-08-25 Published:2013-08-25
  • Contact: Esmaeil Biazar, Ph.D., Department of Biomedical Engineering, Tonekabon branch, Islamic Azad University, Tonekabon, Iran, kia_esm@yahoo.com.
  • Supported by:

    伊朗伊斯兰阿扎德大学基金项目（No. 73/442453）

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摘要：

纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管已被证实可促进大鼠周围神经再生，但其用于修复大于30mm的长段周围神经缺损的效果尚有待评估。实验用纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管桥接大鼠30 mm长的坐骨神经缺损段。4个月后，大体观察和组织学评估发现坐骨神经有再生现象发生；纳米纤维导管内的坐骨神经连续，可见有髓鞘神经纤维的形成，再生的神经中出现许旺细胞和胶质细胞。Masson’s三色染色显示，移植纳米纤维导管的腓肠肌肌细胞无病理改变。结果说明纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管可用于修复长段坐骨神经缺损。

关键词: 神经再生, 周围神经损伤, 坐骨神经, 人工神经导管, 纳米纤维, 生物材料, 大体观察, 组织学, 基金资助文章

Abstract:

It has been confirmed that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit can promote peripheral nerve regeneration in rats. However, its efficiency in repair of over 30-mm-long sciatic nerve defects needs to be assessed. In this study, we used a nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit to bridge a 30-mm-long gap in the rat sciatic nerve. At 4 months after nerve conduit implantation, regenerated nerves were macroscopi-cally observed and histologically assessed. In the nanofibrous graft, the rat sciatic nerve trunk had been reconstructed by restoration of nerve continuity and formation of myelinated nerve fiber. There were Schwann cells and glial cells in the regenerated nerves. Masson’s trichrome staining showed that there were no pathological changes in the size and structure of gastrocnemius muscle cells on the operated side of rats. These findings suggest that nanofibrous poly(3-hydroxybutyrate-co-3- hydroxyvalerate) nerve conduit is suitable for repair of long-segment sciatic nerve defects.

Key words: neural regeneration, peripheral nerve injury, sciatic nerve, artificial conduit, nanofiber, poly(3-hydr- oxybutyrate-co-3-hydroxyvalerate), macroscopic observation, histology, grants-supported paper, neuroregeneration