中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (14): 1351-1357.doi: 10.4103/1673-5374.137587

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

翻转静脉导管填充富血小板血浆提高修复短距离损伤坐骨神经的效果

  

  • 收稿日期:2014-06-12 出版日期:2014-07-25 发布日期:2014-07-25

An inside-out vein graft filled with platelet-rich plasma for repair of a short sciatic nerve defect in rats

Ji Yeong Kim 1, Woo Joo Jeon 2, Dong Hwee Kim 3, Im Joo Rhyu 4, Young Hwan Kim 2, Inchan Youn 5, Jong Woong Park 2   

  1. 1 Department of Orthopedic Surgery, Seoul Paik Hospital, Inje University, Seoul, Korea
    2 Department of Orthopedic Surgery, Korea University, Seoul, Korea
    3 Department of Physical Medicine & Rehabilitation, Korea University, Seoul, Korea
    4 Department of Anatomy, Korea University, Seoul, Korea
    5 Biomedical Research Center, Korea Institute of Science and Technology, Seoul, Korea
  • Received:2014-06-12 Online:2014-07-25 Published:2014-07-25
  • Contact: Jong Woong Park, M.D., Korea University Ansan Hospital, Gojan 1-Dong, Danwon-Gu, Ansan-Si, Gyeonggi-Do, 152-703, Korea, ospark@korea.ac.kr.

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摘要：

富含多种生长因子的富血小板血浆有促进神经再生作用。翻转静脉移植物可代替自体神经移植物修复短距离神经缺损。实验假设翻转静脉移植物填充富血小板血浆后，修复大鼠缺损坐骨神经效果更好。结果显示，填充富血小板血浆的翻转静脉移植物修复大鼠10mm坐骨神经缺损伤后：（1）大鼠坐骨神经功能明显较单纯翻转静脉移植物改善，甚至在6和8周时，坐骨神经功能较自体坐骨神经移植更好；（2）坐骨神经有髓轴突数量多于单纯翻转静脉移植物；轴突直径和髓鞘厚度较单纯翻转静脉移植物大，与自体坐骨神经移植相近。说明填充富血小板血浆的翻转静脉移植物有潜力代替自体神经修复坐骨神经短距离缺损。

关键词: 神经再生, 周围神经损伤, 坐骨神经, 富血小板血浆, 翻转静脉, 有髓轴突, 轴突直径, 髓鞘厚度, 组织学, 坐骨神经指数

Abstract:

Platelet-rich plasma containing various growth factors can promote nerve regeneration. An inside-out vein graft can substitute nerve autograft to repair short nerve defects. It is hypothesized that an inside-out vein graft filled with platelet-rich plasma shows better effects in the repair of short sciatic nerve defects. In this study, an inside-out vein autograft filled with platelet-rich plasma was used to bridge a 10 mm-long sciatic nerve defect in rats. The sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better improved than that of rats with a simple inside-out vein autograft. At 6 and 8 weeks, the sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better than that of rats undergoing nerve autografting. Compared with the sciatic nerve repaired with a simple inside-out vein autograft, the number of myelinated axons was higher, axon diameter and myelin sheath were greater in the sciatic nerve repaired with an inside-out vein autograft filled with platelet-rich plasma and they were similar to those in the sciatic nerve repaired with nerve autograft. These findings suggest that an inside-out vein graft filled with platelet-rich plasma can substitute nerve autograft to repair short sciatic nerve defects.

Key words: nerve regeneration, peripheral nerve injury, sciatic nerve, platelet-rich plasma, inside-out vein autograft, myelinated axons, axon diameter, myelin sheath thickness, histology, sciatic nerve index, neural regeneration