中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (14): 1358-1364.doi: 10.4103/1673-5374.137588

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

化学脱细胞同种异体神经复合睫状神经营养因子可更好促进坐骨神经修复

  

  • 收稿日期:2014-06-28 出版日期:2014-07-25 发布日期:2014-07-25

Chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor promotes sciatic nerve repair

Yanru Zhang 1, 2, Hui Zhang 3, Kaka Katiella 4, Wenhua Huang 2   

  1. 1 Institute of International Education, Zhengzhou University, Zhengzhou, Henan Province, China
    2 Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China
    3 Department of Orthopedics, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China
    4 Institute of Clinical Medicine, Zhengzhou University, Zhengzhou, Henan Province, China
  • Received:2014-06-28 Online:2014-07-25 Published:2014-07-25
  • Contact: Wenhua Huang, Ph.D., Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515, Guangdong Province, China,jiaoxueban2010@126.com.

摘要:

化学脱细胞同种异体神经能减轻移植后的免疫排斥反应,具有与自体神经移植近似的引导神经再生的功能,但复合神经因子后是否可为神经再生提供良好的局部环境?实验以此制备大鼠双侧坐骨神经制备化学去细胞同种异体神经,并复合睫状神经营养因子桥接修复大鼠缺损坐骨神经,设单独化学去细胞同种异体神经桥接组和自体神经翻转吻合组作对照。修复后8周,观察大鼠坐骨神经指数、比目鱼肌诱发电位波幅、三头肌湿质量恢复率、髓鞘厚度、有髓神经纤维数量,在3组中为自体神经翻转吻合组>去细胞同种异体神经+睫状神经营养因子组>去细胞同种异体神经桥接组。说明化学脱细胞同种异体神经复合睫状神经营养因子可修复缺损坐骨神经,虽修复效果不及自体神经翻转吻合,但优于单独应用化学脱细胞同种异体神经桥接法。

关键词: 神经再生, 周围神经损伤, 化学脱细胞同种异体神经, 缺损, 修复, 移植, 睫状神经营养因子, 自体神经

Abstract:

A chemically extracted acellular allogeneic nerve graft can reduce postoperative immune rejection, similar to an autologous nerve graft, and can guide neural regeneration. However, it remains poorly understood whether a chemically extracted acellular allogeneic nerve graft combined with neurotrophic factors provides a good local environment for neural regeneration. This study investigated the repair of injured rat sciatic nerve using a chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor. An autologous nerve anastomosis group and a chemical acellular allogeneic nerve bridging group were prepared as controls. At 8 weeks after repair, sciatic functional index, evoked potential amplitude of the soleus muscle, triceps wet weight recovery rate, total number of myelinated nerve fibers and myelin sheath thickness were measured. For these indices, values in the three groups showed the autologous nerve anastomosis group > chemically extracted acellular nerve graft + ciliary neurotrophic factor group > chemical acellular allogeneic nerve bridging group. These results suggest that chemically extracted acellular nerve grafts combined with ciliary neurotrophic factor can repair sciatic nerve defects, and that this repair is inferior to autologous nerve anastomosis, but superior to chemically extracted acellular allogeneic nerve bridging alone.

Key words: nerve regeneration, peripheral nerve injury, chemically extracted acellular allogeneic nerve, defect, repair, transplantation, ciliary neurotrophic factor, autologous nerve, neural regeneration