中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (20): 1824-1829.doi: 10.4103/1673-5374.143430

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

三维适形调强放疗左侧股骨病灶：放疗后坐骨神经未受到损伤

  

  • 收稿日期:2014-07-08 出版日期:2014-10-25 发布日期:2014-10-25
  • 基金资助:

    国家自然科学基金项目（81360276）；新疆维吾尔自治区科技支疆项目（2013911129）；新疆维吾尔自治区青年科技创新人才培养工程项目-青年博士科技人才培养项目（2013731013）；新疆维吾尔自治区自然科学基金项目（2012211B34）；中国博士后科学基金（2014M552566XB）

Three-dimensional conformal intensity-modulated radiation therapy of left femur foci does not damage the sciatic nerve

Wanlong Xu 1, Xibin Zhao 1, Qing Wang 1, Jungang Sun 1, Jiangbo Xu 1, Wenzheng Zhou 1, Hao Wang 1, Shigui Yan 2, Hong Yuan 1   

  1. 1 People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China
    2 The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China
  • Received:2014-07-08 Online:2014-10-25 Published:2014-10-25
  • Contact: Hong Yuan, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830011, Xinjiang Uygur Autonomous Region, China, Doctoryuanhong@sina.com. Shigui Yan, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China, zrjwsj@zju.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81360276; a grant from the Science and Technology Project of Xinjiang Uygur Autonomous Region in China, No. 2013911129; a grant from the Youth Science and Technology Innovation Talents Project of Xinjiang Uygur Autonomous Region-Young Doctor Talents Training Project, No. 2013731013; the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2012211B34; the China Postdoctoral Science Foundation, No. 2014M552566XB.

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摘要：

用放射治疗杀灭股骨棘球蚴病虫体过程中，其病灶周围的坐骨神经容易受到放疗损伤，因此评估放疗对周围神经的安全性至关重要。实验采用三维适形调强放疗治疗双侧股骨棘球蚴病子午沙鼠，左侧股骨棘球蚴病灶给予40Gy的放射治疗14d，右侧股骨给予假照射。以HE染色、电镜和TUNEL法观察到子午沙鼠左侧坐骨神经神经元结构正常，无明显凋亡迹象；以锥虫蓝染色观察到子午沙鼠左侧股骨的骨细粒棘球蚴原头节整体结构消失，原头节死亡率高于右侧，酶化学染色法结果显示左侧股骨的骨细粒棘球蚴原头节的琥珀酸脱氢酶活性较右侧减弱。结果证实三维适形调强放疗对子午沙鼠继发性骨棘球蚴病有很好的治疗效果，且坐骨神经形态和功能未受到放射线损伤。

关键词: 神经再生, 三维适形调强放疗, 棘球蚴病, 坐骨神经, 神经元, 放射损伤, 琥珀酸脱氢酶, NSFC grant

Abstract:

During radiotherapy to kill femoral hydatid tapeworms, the sciatic nerve surrounding the focus can be easily damaged by the treatment. Thus, it is very important to evaluate the effects of radiotherapy on the surrounding nervous tissue. In the present study, we used three-dimensional, conformal, intensity-modulated radiation therapy to treat bilateral femoral hydatid disease in Meriones meridiani. The focus of the hydatid disease on the left femur was subjected to radiotherapy (40 Gy) for 14 days, and the right femur received sham irradiation. Hematoxylin-eosin staining, electron microscopy, and terminal deoxynucleotidyl transferase-dUTP nick end labeling assays on the left femurs showed that the left sciatic nerve cell structure was normal, with no obvious apoptosis after radiation. Trypan blue staining demonstrated that the overall protoscolex structure in bone parasitized with Echinococcus granulosus disappeared in the left femur of the animals after treatment. The mortality of the protoscolex was higher in the left side than in the right side. The succinate dehydrogenase activity in the protoscolex in bone parasitized with Echinococcus granulosus was lower in the left femur than in the right femur. These results suggest that three-dimensional conformal intensity-modulated radiation therapy achieves good therapeutic effects on the secondary bone in hydatid disease in Meriones meridiani without damaging the morphology or function of the sciatic nerve.

Key words: nerve regeneration, three-dimensional conformal intensity-modulated radiation therapy, hydatid disease, sciatic nerve, neurons, radiation damage, succinate dehydrogenase, NSFC grants, neural regeneration