中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (3): 394-403.doi: 10.4103/1673-5374.153687

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

电针修复脊髓损伤:抑制Notch信号通路与促进神经干细胞增殖?

  

  • 收稿日期:2015-02-04 出版日期:2015-03-20 发布日期:2015-03-20
  • 基金资助:

    云南省教育厅科学研究基金重大专项资金(zd2012001)

Electroacupuncture in the repair of spinal cord injury: inhibiting the Notch signaling pathway and promoting neural stem cell proliferation

Xin Geng, Tao Sun, Jing-hui Li, Ning Zhao, Yong Wang, Hua-lin Yu   

  1. Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
  • Received:2015-02-04 Online:2015-03-20 Published:2015-03-20
  • Contact: Hua-lin Yu, M.D., yuhualinbio@126.com
  • Supported by:

    This work was supported by the Major Special Project of Scientific Research Fund of Yunnan Provincial Education Department of China, No. zd2012001

摘要:

电针督脉治疗脊髓损伤有较好的临床疗效,但机制尚不明确。实验给予成年SD大鼠脊髓夹击损伤60 s,次日电针大椎穴(GV14)和命门穴(GV4)。酶联免疫吸附检测发现电针后脊髓损伤模型大鼠血清炎症因子表达明显下调,苏木精-伊红染色和免疫组织化学结果显示电针可促进大鼠损伤脊髓组织神经干细胞的增殖,并抑制其向星型胶质细胞分化,实时荧光定量PCR和Western blot检测,见电针抑制了脊髓损伤诱导的Notch信号通路的激活。结果证实电针通过抑制Notch信号通路,促进内源性神经干细胞增殖,进而达到修复损伤脊髓的目的

关键词: 神经再生, 脊髓, 电针治疗, 神经干细胞, Notch信号通路, 星形胶质细胞, 炎症, 生存曲线, 增殖, 分化, 实时荧光定量PCR, 蛋白印迹

Abstract:

Electroacupuncture for the treatment of spinal cord injury has a good clinical curative effect, but the underlying mechanism is unclear. In our experiments, the spinal cord of adult Sprague-Dawley rats was clamped for 60 seconds. Dazhui (GV14) and Mingmen (GV4) acupoints of rats were subjected to electroacupuncture. Enzyme-linked immunosorbent assay revealed that the expression of serum inflammatory factors was apparently downregulated in rat models of spinal cord injury after electroacupuncture. Hematoxylin-eosin staining and immunohistochemistry results demonstrated that electroacupuncture contributed to the proliferation of neural stem cells in rat injured spinal cord, and suppressed their differentiation into astrocytes. Real-time quantitative PCR and western blot assays showed that electroacupuncture inhibited activation of the Notch signaling pathway induced by spinal cord injury. These findings indicate that electroacupuncture repaired the injured spinal cord by suppressing the Notch signaling pathway and promoting the proliferation of endogenous neural stem cells.

Key words: nerve regeneration, spinal cord, electroacupuncture therapy, neural stem cells, notch signaling pathway, astrocytes, inflammation, survival curve, proliferation, differentiation, real-time quantitative PCR, western blot assay, neural regeneration