中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (4): 657-663.doi: 10.4103/1673-5374.180754

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

远志皂苷元可抑制挫伤脊髓的神经元凋亡

  

  • 收稿日期:2015-12-22 出版日期:2016-04-30 发布日期:2016-04-30
  • 基金资助:

    基金资助：吉林省科技发展计划资助项目（2011084）

Senegenin inhibits neuronal apoptosis after spinal cord contusion injury

Shu-quan Zhang1, Min-fei Wu2, Rui Gu3, Jia-bei Liu3, Ye Li3, Qing-san Zhu3, Jin-lan Jiang3, 4, *   

  1. 1 Department of Orthopedics, Nankai Hospital, Tianjin, China
    2 Department of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China
    3 Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
    4 Scientific Research Center, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
  • Received:2015-12-22 Online:2016-04-30 Published:2016-04-30
  • Contact: Jin-lan Jiang, 3213767434@qq.com.
  • Supported by:

    This research was supported by a grant from the Science and Technology Development Plan of Jilin Province of China, No. 2011084.

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摘要：

远志皂甙元对神经元凋亡有明显抑制作用，从而起到了神经保护作用。实验按照改良Allen打击法建立了急性脊髓挫伤大鼠模型，损伤3h后，连续3d尾静脉注射注远志皂苷元30mg/g，可见大鼠脊髓空洞缩小，凋亡细胞数量明显减少，脊髓损伤区Bax、Caspase-3 mRNA及蛋白表达水平减少，Bcl-2 mRNA及蛋白表达水平增加，近脑端脊髓中神经纤维密度增加，后肢运动和电生理及神经功能明显恢复。结果表明远志皂苷元通过抑制脊髓挫伤区神经元凋亡对脊髓挫伤起到了保护作用。

orcid: 0000-0002-8777-4783 (Jin-lan Jiang)

关键词: 神经再生, 脊髓挫伤, 远志皂苷元, 远志, 运动功能, 细胞凋亡, 电生理

Abstract:

Senegenin has been shown to inhibit neuronal apoptosis, thereby exerting a neuroprotective effect. In the present study, we established a rat
model of spinal cord contusion injury using the modified Allen’s method. Three hours after injury, senegenin (30 mg/g) was injected into
the tail vein for 3 consecutive days. Senegenin reduced the size of syringomyelic cavities, and it substantially reduced the number of apoptotic
cells in the spinal cord. At the site of injury, Bax and Caspase-3 mRNA and protein levels were decreased by senegenin, while Bcl-2
mRNA and protein levels were increased. Nerve fiber density was increased in the spinal cord proximal to the brain, and hindlimb motor
function and electrophysiological properties of rat hindlimb were improved. Taken together, our results suggest that senegenin exerts a
neuroprotective effect by suppressing neuronal apoptosis at the site of spinal cord injury.

Key words: nerve regeneration, spinal cord contusion, senegenin, thinleaf milkwort root, motor function, apoptosis, electrophysiology, neural regeneration