中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (12): 2751-2756.doi: 10.4103/1673-5374.373718

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

一种新型颈脊髓中央管综合征的模型

  

  • 出版日期:2023-12-15 发布日期:2023-06-15
  • 基金资助:
    干细胞与转化研究国家重点研发项目(2019YFA0112100);国家自然科学基金(重点项目)(81930070)

A novel mouse model of central cord syndrome

Elzat Elham – Yilizati Yilihamu1, #, Xiangchuang Fan1, #, Zimeng Yang1, Shiqing Feng1, 2, *   

  1. 1Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, Shandong Province, China; 2Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China
  • Online:2023-12-15 Published:2023-06-15
  • Contact: Shiqing Feng, MD, PhD, shiqingfeng@sdu.edu.cn.
  • Supported by:
    This study was supported by the National Key Research and Development Project of Stem Cell and Transformation Research, No. 2019YFA0112100; and the National Natural Science Foundation of China (Key Program), No. 81930070 (both to SF).

摘要:

椎管狭窄患者容易受到钝性损伤而诱发中央管综合征,寻找合适的动物模型有助于研究此类损伤的发病机制和治疗方法。实验基于小鼠颈部解剖结构,以5和10g/mm2压缩重量压迫C6脊髓建立急性钝性创伤性脊髓损伤模型,以模拟颈脊髓中央管综合征。行为学检测证实该模型符合颈脊髓中央管综合征特征,即前肢运动功能明显受损,而后肢基本上保留了运动和感觉功能。进一步苏木精-伊红染色发现,模型小鼠的脊髓病变区域都位于中央管附近的灰质,而外侧白质几乎未见损伤。核磁共振成像观察可见,轻度和重度损伤后,脊髓病变中心存在明显的低信号区。另外,免疫荧光染色提示脊髓白质区神经束损伤较小,且持续存在慢性炎症反应。实验结果提示,这种新型中央管综合征小鼠模型可作为临床前研究的模型,且中央管综合征模型中灰质是最易受影响的区域,会影响其支配的运动功能。

https://orcid.org/0000-0002-5939-138X (Shiqing Feng)

关键词: 脊髓, 脊髓损伤, 脊髓压迫, 颈脊髓, 颈髓损伤, 中央管综合征, 动物模型, 临床前研究, 行为学, 病理学

Abstract: Patients with potential spinal stenosis are susceptible to central cord syndrome induced by blunt trauma. Suitable animal models are helpful for studying the pathogenesis and treatment of such injuries. In this study, we established a mouse model of acute blunt traumatic spinal cord injury by compressing the C6 spinal cord with 5 and 10 g/mm2 compression weights to simulate cervical central cord syndrome. Behavioral testing confirmed that this model exhibited the characteristics of central cord syndrome because motor function in the front paws was impaired, whereas basic motor and sensory functions of the lower extremities were retained. Hematoxylin-eosin staining showed that the diseased region of the spinal cord in this mouse model was restricted to the gray matter of the central cord, whereas the white matter was rarely affected. Magnetic resonance imaging showed a hypointense signal in the lesion after mild and severe injury. In addition, immunofluorescence staining showed that the degree of nerve tract injury in the spinal cord white matter was mild, and that there was a chronic inflammation reaction. These findings suggest that this mouse model of central cord syndrome can be used as a model for preclinical research, and that gray matter is most vulnerable to injury in central cord syndrome, leading to impaired motor function.

Key words: animal model, behavior, central cord syndrome, cervical spinal cord injury, cervical spinal cord, pathology, preclinical research, spinal cord, spinal cord compression, spinal cord injury