中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (1): 60-64.doi: 10.4103/1673-5374.150707

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

构建更接近人类马尾神经损伤修复的大动物模型:山羊模型外科修复后的评价

  

  • 收稿日期:2014-12-15 出版日期:2015-01-15 发布日期:2015-01-15
  • 基金资助:

    中国国家科技部973项目计划(批准号:2014cb542200),教育部创新团队计划(irt1201)

Large animal models of human cauda equina injury and repair: evaluation of a novel goat model

Wen-tao Chen 1, Pei-xun Zhang 1, *, Feng Xue 1, *, Xiao-feng Yin 1, Cao-yuan Qi 2, Jun Ma 2, Bo Chen 1, You-lai Yu 1, Jiu-xu Deng 1, Bao-guo Jiang 1, *   

  1. 1 Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China
    2 Department of Radiology, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China
  • Received:2014-12-15 Online:2015-01-15 Published:2015-01-15
  • Contact: Pei-xun Zhang, M.D., Ph.D., zhangpeixun@126.com. Feng Xue, M.D., Ph.D., drxuefeng@gmail.com. Bao-guo Jiang, M.D., Ph.D., jiangbaoguo@vip.sina.com.
  • Supported by:

    This study was supported by grants from the National Program on Key Basic Research Project of China (973 Program), No. 2014CB542200; Program for Innovative Research Team in University of Ministry of Education of China, No. IRT1201; the National Natural Science Foundation of China, No. 31271284, 31171150, 81171146, 30971526, 31040043, 31371210, 81372044, 31471144; Program for New Century Excellent Talents in University of Ministry of Education of China, No. BMU20110270; and the Natural Science Foundation of Beijing of China, No. 7142164.

摘要:

以往有关马尾神经损伤研究的动物模型多见于大鼠,与人类损伤后情况差异较大,并且多数研究偏重于电生理方面。为了更好地模拟人类马尾神经损伤后的外科修复效果,我们建立了马尾神经损伤修复山羊模型,用电生理、组织学和磁共振方法评价马尾神经损伤及断端缝合后的形态学和功能性恢复效果。作者认为,实验构建的马尾神经损伤修复山羊模型,能够提供更模拟人类马尾神经损伤外科修复后再生过程的详细信息。

关键词: 神经再生, 脊髓损伤, 山羊, 动物模型, 放射摄影术, 磁共振成像, DTI弥散张量成像, 纤维束, 诊断, 损伤, 生理学, 神经影像学

Abstract:

Previous animal studies of cauda equina injury have primarily used rat models, which display significant differences from humans. Furthermore, most studies have focused on electrophysiological examination. To better mimic the outcome after surgical repair of cauda equina injury, a novel animal model was established in the goat. Electrophysiological, histological and magnetic resonance imaging methods were used to evaluate the morphological and functional outcome after cauda equina injury and end-to-end suture. Our results demonstrate successful establishment of the goat experimental model of cauda equina injury. This novel model can provide detailed information on the nerve regenerative process following surgical repair of cauda equina injury.

Key words: nerve regeneration, spinal cord injury, goat, animal model, radiography, magnetic , resonance imaging, diffusion tensor imaging, fiber bundle, diagnosis, injury, physiology, neuroimaging, NSFC grants, neural regeneration