中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (12): 2344-2352.doi: 10.4103/1673-5374.284998

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

枸杞多糖神经保护作用与调节视网膜胶质细胞的反应性有关

  

  • 出版日期:2020-12-15 发布日期:2020-08-05
  • 基金资助:
    国家基础研究计划项目（81300766），暨南大学附属第一医院培养与创新基金（802168）；广东省卫生卫生技术推广计划项目（201905270933056876）；广东省领军人才基金（87014002）；宁夏重点研究发展计划和中国高校学科引进人才计划资助项目（B14036）

Lycium barbarum polysaccharides related RAGE and Aβ levels in the retina of mice with acute ocular hypertension and promote maintenance of blood retinal barrier

Xue-Song Mi 1, 3 , Qian Feng 2, 4, 7 , Amy Cheuk Yin Lo 5 , Raymond Chuen-Chung Chang 4, 7 , Sookja Kim Chung 6, 7 , Kwok-Fai So 2, 3, 4, 5   

  1. 1 Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China
    2 Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, Guangdong Province, China
    3 Changsha Academician Expert Workstation, Aier Eye Hospital Group, Changsha, Hunan Province, China
    4 State Key Laboratory of Brain and Cognitive Sciences, Hong Kong Special Administrative Region, China
    5 Department of Ophthalmology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    6 Faculty of Medicine, Macau University of Science and Technology, Macao Special Administrative Region, China
    7 School of Biomedical Science, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
  • Online:2020-12-15 Published:2020-08-05
  • Contact: Kwok-Fai So, PhD, hrmaskf@hku.hk.
  • Supported by:
    This study was supported in part by the National Basic Research Program of China, No. 81300766 (to XSM); the Cultivation and Innovation Fund from the First Affiliated Hospital of Jinan University, China, No. 802168 (to XSM); Hygiene & Health Appropriated Technology and Promoting Project of Guangdong Province of China, No. 201905270933056876 (to XSM); the fund of Leading Talents of Guangdong Province of China, No. 87014002 (to KFS); a grant from Ningxia Key Research and Development Program, and Programme of Introducing Talents of Discipline to Universities of China, No. B14036 (to KFS).

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摘要：

作者既往研究已证实，枸杞多糖对急性眼高压小鼠视网膜神经元和血管存在保护作用。为进一步说明枸杞多糖对急性高眼压损伤视网膜胶质细胞反应性的影响，实验以维持90 mmHg高眼压60min的方法建立急性高眼压模型，于损伤前7d至损伤后4d每天灌胃枸杞多糖1mg/kg，发现急性高眼压损伤小鼠视网膜星形胶质细胞和小胶质细胞数量增加，视网膜中神经胶质细胞生物标志物胶质纤维酸性蛋白、谷氨酰胺合成酶、S-100、水通道蛋白4和Iba-1以及淀粉样前体蛋白和糖基化终末产物受体表达异常升高；而经枸杞多糖干预后，上述变化明显恢复，还可见胶质纤维酸性蛋白标记的与血管相关的视网膜星形胶质细胞的形态重塑。因此，作者认为枸杞多糖可通过调节淀粉样前体蛋白的产生和糖基化终末产物的表达，以及对视网膜胶质细胞活性的提高，在急性高眼压损伤过程中维持血视网膜屏障和神经元的活性。实验于2008年1月1日经活体动物教学和研究委员会批准（批准号CULTRA-＃1664-08）。

orcid: 0000-0003-4039-4246 (Kwok-Fai So)

关键词: 眼, 视神经, 视网膜, 模型, 枸杞, 胶质细胞, 网膜屏障, 再生, 可塑性

Abstract: Our previous study verified the protective effects of Lycium barbarum polysaccharides (LBP) on retinal neurons and blood vessels in acute ocular hypertension (AOH) mice. To investigate the effect of LBP on the reactivity of retinal glial cells, an AOH mouse model was estab- lished in one eye by maintaining ocular hypertension of 90 mmHg for 60 minutes. Either LBP solution (1 mg/kg) or phosphate-buffered saline was administrated to the mice by gavage daily, starting 7 days before the AOH insult and continuing until the mice were sacrificed for specimen collection on day 4 post-insult. After AOH insult, increased numbers of astrocytes and microglia were observed, together with decreased expression of the following glial cell biomarkers in the retinal ganglion cells of AOH mice: glial fibrillary acidic protein, glutamine synthetase, aquaporin-4, S-100 proteins, ionized calcium-binding adaptor molecule 1, amyloid precursor protein and receptor of advanced glycosylation end-products. After intervention with LBP, the above changes were significantly reduced. Remarkably, morpho- logical remodeling of blood vessel-associated retinal astrocytes, marked by glial fibrillary acidic protein, was also observed. These results, taken together, suggest that LBP regulated the production of amyloid-β and expression of receptor of advanced glycosylation end-products, as well as mediating the activity of retinal glial cells, which may lead to the promotion of better maintenance of the blood-retinal barrier and improved neuronal survival in AOH insult. This study was approved by the Committee for the Use of Live Animals in Teaching and Research (approval No. CULTRA-#1664-08).

Key words: astrocyte, blood-retinal barrier, glial cell, Lycium barbarum, microglia, model, plasticity, remodel, retina