中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (6): 1343-1347.doi: 10.4103/1673-5374.327351

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

髓鞘相关糖蛋白联合几丁质导管神经帽抑制坐骨神经横断后疼痛性神经瘤的形成

  

  • 出版日期:2022-06-15 发布日期:2021-12-17

Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection

Wei Pi1, 2, 3, Ci Li1, 2, 3, Meng Zhang1, 2, 3, Wei Zhang1, 2, 3, Pei-Xun Zhang1, 2, 3, *   

  1. 1Department of Orthopedics and Trauma, Peking University People’s Hospital, Peking University, Beijing, China; 2Key Laboratory of Trauma and Neural Regeneration, Peking University, Beijing, China; 3National Center for Trauma Medicine, Beijing, China
  • Online:2022-06-15 Published:2021-12-17
  • Contact: Pei-Xun Zhang, PhD, zhangpeixun@bjmu.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 31771322; Major R&D Program of National Ministry of Science and Technology of China, No. 2018YFB1105504; Beijing Natural Science Foundation of China, No. 7212121; and Shenzhen Science and Technology Plan Project of China, No. JCYJ20190806162205278 (all to PXZ).

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摘要：

有研究显示，髓鞘相关糖蛋白可抑制神经损伤后轴突的再生，然而髓鞘相关糖蛋白对周围神经损伤后神经瘤形成的影响尚不清楚。因此，实验以髓鞘相关糖蛋白局部注射联合几丁质导管制作的神经帽干预坐骨神经横断后疼痛性神经瘤的形成。结果发现，联合治疗8周后，坐骨神经横断大鼠的自伤行为减少，且近端神经残端中疼痛标志物神经生长因子mRNA表达减低，再生轴突密度减少，髓鞘厚度增加，无髓与有髓轴突比例减少，同时近端神经残端中胶原纤维面积百分比和纤维化标志物α-平滑肌肌动蛋白阳性染色面积百分比降低，且上述作用效果优于单独几丁质导管治疗。表明髓鞘相关糖蛋白联合几丁质导管可协同抑制坐骨神经横断后疼痛性神经瘤的形成，并缓解神经性疼痛。实验于2019年12月5日经北京大学人民医院动物伦理委员会批准（批准号2019PHE027）。

https://orcid.org/0000-0001-7200-2281 (Pei-Xun Zhang)

关键词: 疼痛性神经瘤, 周围神经, 髓鞘相关糖蛋白, 几丁质导管, 再生, 自伤, 轴突, 纤维化

Abstract: Studies have shown that myelin-associated glycoprotein (MAG) can inhibit axon regeneration after nerve injury. However, the effects of MAG on neuroma formation after peripheral nerve injury remain poorly understood. In this study, local injection of MAG combined with nerve cap made of chitin conduit was used to intervene with the formation of painful neuroma after sciatic nerve transfection in rats. After 8 weeks of combined treatment, the autotomy behaviors were reduced in rats subjected to sciatic nerve transfection, the mRNA expression of nerve growth factor, a pain marker, in the proximal nerve stump was decreased, the density of regenerated axons was decreased, the thickness of the myelin sheath was increased, and the ratio of unmyelinated to myelinated axons was reduced. Moereover, the percentage of collagen fiber area and the percentage of fibrosis marker alpha-smooth muscle actin positive staining area in the proximal nerve stump were decreased. The combined treatment exhibited superior effects in these measures to chitin conduit treatment alone. These findings suggest that MAG combined with chitin conduit synergistically inhibits the formation of painful neuroma after sciatic nerve transection and alleviates neuropathic pain. This study was approved by the Animal Ethics Committee of Peking University People’s Hospital (approval No. 2019PHE027) on December 5, 2019.

Key words: autotomy, axon, chitin conduit, fibrosis, myelin-associated glycoprotein, painful neuroma, peripheral nerve, regeneration

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