中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (9): 783-791.doi: 10.3969/j.issn.1673-5374.2013.09.002

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

茶多酚保护全脑缺血再灌注损伤的最佳剂量

  

  • 收稿日期:2012-12-27 修回日期:2013-01-30 出版日期:2013-03-25 发布日期:2013-03-25
  • 基金资助:

    国家自然科学基金(81071070)

An optimal dose of tea polyphenols protects against global cerebral ischemia/reperfusion injury

Jianrui Lv, Rongliang Xue, Jing Zhao, Xin Wei, Hui Gao, Rongguo Fu, Gang Wu, Wei Li, Xiaoming Lei, Junbin Tian   

  1. Department of Anesthesiology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2012-12-27 Revised:2013-01-30 Online:2013-03-25 Published:2013-03-25
  • Contact: Rongliang Xue, Master, Professor, Department of Anesthesiology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China, xuerl299@163.com.
  • About author:Jianrui Lv★, Master, Associate professor.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81071070.

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摘要：

以往对茶多酚保护脑缺血再灌注损伤的研究多采用局灶性脑缺血再灌注模型，但其最佳保护剂量尚不统一。鉴于此，实验采用4动脉阻断法建立全脑缺血再灌注损伤大鼠模型，用不同剂量茶多酚(25，50，100，150，200 mg/kg)腹腔注射干预。结果发现再灌注2，6，12，24，48和72 h，茶多酚各剂量干预组脑组织过氧化物歧酶活力及总抗氧化能力逐渐增高，丙二醛含量逐渐降低，茶多酚200 mg/kg干预组变化最显著。TUNEL法和流式细胞仪检测结果显示，茶多酚200 mg/kg干预后的脑缺血再灌注大鼠海马CA1区凋亡细胞数量和细胞凋亡率均明显减少。旷场试验和高架十字迷宫实验显示，茶多酚200 mg/kg干预脑缺血再灌注大鼠探索能力增强，焦虑情绪减轻。结果说明，茶多酚对大鼠全脑缺血再灌注损伤有保护作用，最佳保护剂量为200 mg/kg。

关键词: 神经再生, 中医药, 脑损伤, 茶多酚, 脑, 缺血再灌注损伤, 氧化应激, 神经行为学, 凋亡, 基金资助文章, 图片文章

Abstract:

Previous studies addressing the protection of tea polyphenols against cerebral ischemia/ reperfusion injury often use focal cerebral ischemia models, and the optimal dose is not unified. In this experiment, a cerebral ischemia/reperfusion injury rat model was established using a modified four-vessel occlusion method. Rats were treated with different doses of tea polyphenols (25, 50, 100, 150, 200 mg/kg) via intraperitoneal injection. Results showed that after 2, 6, 12, 24, 48 and 72 hours of reperfusion, peroxide dismutase activity and total antioxidant capacity in brain tissue gradually increased, while malondialdehyde content gradually decreased after tea polyphenol intervention. Tea polyphenols at 200 mg/kg resulted in the most apparent changes. Terminal deoxynucleotidyl transferase-mediated nick end labeling and flow cytometry showed that 200 mg/kg tea polyphenols significantly reduced the number and percentage of apoptotic cells in the hippocampal CA1 region of rats after cerebral ischemia/reperfusion injury. The open field test and elevated plus maze experiments showed that tea polyphenols at 200 mg/kg strengthened exploratory behavior and reduced anxiety of cerebral ischemia/reperfusion injured rats. Experimental findings indicate that tea polyphenols protected rats against cerebral ischemia/ reperfusion injury and 200 mg/kg is regarded as the optimal dose.

Key words: neural regeneration, brain injury, traditional Chinese medicine, tea polyphenols, brain, ischemia/reperfusion injury, oxidative stress, neuroethology, apoptosis, grants-supported paper, photographs-containing paper, neuroregeneration