中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (9): 817-824.doi: 10.3969/j.issn.1673-5374.2013.09.006

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

产前感染合并缺氧方法制备脑性瘫痪幼鼠模型

  

  • 收稿日期:2012-11-03 修回日期:2013-01-09 出版日期:2013-03-25 发布日期:2013-03-25

A rat pup model of cerebral palsy induced by prenatal inflammation and hypoxia

Yanrong Hu1, 2, Gang Chen3, Hong Wan4, Zhiyou Zhang5, Hong Zhi3, Wei Liu5, Xinwei Qian3, Mingzhao Chen3, Linbao Wen5, Feng Gao5, Jianxin Li6, Lihui Zhao7   

  1. 1 Postdoctoral Research Station, School of Basic Medicine, CAMA and PUMC, Beijing 100000, China;
    2 Postdoctoral Research Station, the People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region, China
    3 Department of Neurosurgery, the Fourth People’s Hospital of Wuxi (The Fourth Affiliated Hospital of Soochow University), Wuxi 214026, Jiangsu Province, China
    4 Beijing Neurosurgical Institute, Beijing 100050, China
    5 Department of Neurosurgery, Xinjiang Autonomous Region People’s Hospital, Urumqi 830001, Xinjiang Uygur Autonomous Region, China
    6 Department of Neurology, Xinjiang Autonomous Region People’s Hospital, Urumqi 830001, Xinjiang Uygur Autonomous Region, China
    7 Department of Pathology, Xinjiang Autonomous Region People’s Hospital, Urumqi 830001, Xinjiang Uygur Autonomous Region, China
  • Received:2012-11-03 Revised:2013-01-09 Online:2013-03-25 Published:2013-03-25
  • Contact: Gang Chen, M.D., Associate chief physician, Department of Neurosurgery, the Fourth People’s Hospital of Wuxi (the Fourth Affiliated Hospital of Soochow University), Wuxi 214026, Jiangsu Province, China, jhy_501@163.com.
  • About author:Yanrong Hu☆, Ph. D., M.D.

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摘要：

采用单纯感染或缺血缺氧法所制备脑性瘫痪动物模型均不能有效的模拟早产儿脑损伤。实验将受孕17 d的健康Wistar孕鼠进行脂多糖腹腔注射及缺氧干预后，以4周龄幼鼠进行试验，同时设颈动脉结扎+缺氧组及对照组进行对比。足印步态重复间距分析试验、平衡木试验、水迷宫试验、神经电生理试验、病理学检测结果显示，生后4周幼鼠与对照组相比，脂多糖缺氧组和颈动脉结扎+缺氧组幼鼠前后肢足印重复间距增大；通过平衡木潜伏期时间较差；水迷宫定位航行和空间探索记忆能力较差；后肢股四头肌复合动作电位引出最大波幅所需的刺激强度增大；间脑室周围组织神经细胞结构紊乱形态不规则，胞膜部分缺损，胞浆内容物外溢。结果证实，实验利用Wistar孕鼠腹腔注射脂多糖宫内感染合并缺氧干预的方法成功制备了Wistar幼鼠脑性瘫痪模型，方法简单可靠。

关键词: 神经再生, 基础实验, 感染, 缺氧, 缺血, 脂多糖, 动物模型, 脑性瘫痪, 水迷宫试验, 神经电生理, 病理, 透射电镜, 基金资助文章

Abstract:

Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.

Key words: neural regeneration, brain injury, hypoxia, lipopolysaccharide, animal models, cerebral palsy, water maze test, neuroelectrophysiology, histopathology, grants-supported paper, photographs- containing paper, neuroregeneration