中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (23): 2190-2197.doi: 10.3969/j.issn.1673-5374.2013.23.009

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

孕期补充牛磺酸减少宫内生长受限胎鼠脑细胞凋亡

  

  • 收稿日期:2013-03-20 修回日期:2013-03-20 出版日期:2013-08-15 发布日期:2013-08-15

Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction

Jing Liu, Xiaofeng Wang, Ying Liu, Na Yang, Jing Xu, Xiaotun Ren   

  1. Department of Neonatology and NICU of Bayi Children’s Hospital, General Hospital of Beijing Military Command, Beijing 100700, China
  • Received:2013-03-20 Revised:2013-03-20 Online:2013-08-15 Published:2013-08-15
  • Contact: Jing Liu, M.D., Ph.D., Chief physician, Professor, Department of Neonatology and NICU of Bayi Children’s Hospital, General Hospital of Beijing Military Command, Beijing 100700, China, liujingbj@live.cn.
  • Supported by:

    国家自然科学基金资助项目（81170577）

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摘要：

自SD大鼠妊娠开始直至分娩，每日给予低蛋白饮食饲养建立乳鼠宫内生长受限模型，自妊娠第12日开始每天于孕鼠饲料中添加300mg/kg牛磺酸直至自然分娩。取分娩后6h的正常乳鼠、宫内生长受限乳鼠及牛磺酸干预的宫内生长受限乳鼠脑组织用于实验。TUNEL染色显示，宫内生长受限乳鼠脑组织凋亡细胞明显增多，而孕鼠补充牛磺酸可减少宫内生长受限乳鼠脑组织细胞凋亡。免疫组织化学染色显示孕鼠补充牛磺酸可促进宫内生长受限胎鼠大脑皮质胶质细胞源性神经营养因子的表达，减少Caspase-3的表达。提示孕鼠补充牛磺酸可通过胶质细胞源性神经营养因子-Caspase-3信号途径减少宫内生长受限胎鼠脑细胞凋亡，从而对宫内生长受限胎鼠发挥脑保护作用。

关键词: 宫内生长受限, 胎鼠, 脑, 神经细胞, 牛磺酸, 细胞凋亡, 胶质细胞源性神经营养因子, Caspase-3, 神经发育, 神经再生

Abstract:

From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added to food until spontaneous delivery occurred. Brain tissues from normal neonatal rats at 6 hours after delivery, neonatal rats with intrauterine growth restriction, and neo-natal rats with intrauterine growth restriction undergoing taurine supplement were obtained for fur-ther experiments. The terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling assay revealed that the number of apoptotic cells in the brain tissue of neonatal rats with intrauterine growth restriction significantly increased. Taurine supplement in pregnant rats reduced cell apoptosis in brain tissue from neonatal rats with intrauterine growth restriction. Immunohistochemical staining revealed that taurine supplement increased glial cell line-derived neurotrophic factor expression and decreased caspase-3 expression in the cerebral cortex of intra-uterine growth-restricted fetal rats. These results indicate that taurine supplement reduces cell apoptosis through the glial cell line-derived neurotrophic factor-caspase-3 signaling pathway, re-sulting in a protective effect on the intrauterine growth-restricted fetal rat brain.

Key words: neural regeneration, intrauterine growth restriction, fetal rats, brain, neural cells, taurine, cell apop-tosis, glial cell line-derived neurotrophic factor, caspase-3, neural development, grants-supported paper, neuroregeneration