中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (30): 2820-2826.doi: 10.3969/j.issn.1673-5374.2013.30.004

• 原著:退行性病与再生 • 上一篇    下一篇

补肾中药抑制多巴胺能神经细胞凋亡的方式

  

  • 收稿日期:2013-02-05 修回日期:2013-04-20 出版日期:2013-10-25 发布日期:2013-10-25

How do Chinese medicines that tonify the kidney inhibit dopaminergic neuron apoptosis?

Shaogang Lin1, Shuifen Ye2, Jinmu Huang1, Yun Tian3, Yihui Xu2, Mengqi Wu2, Jingxia Wang2, Songying Wu4, Jing Cai2   

  1. 1 Hospital of Putian University, Putian 351100, Fujian Province, China
    2 Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
    3 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
    4 The Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
  • Received:2013-02-05 Revised:2013-04-20 Online:2013-10-25 Published:2013-10-25
  • Contact: Jing Cai, M.D., Ph.D., Professor, Doctoral supervisor, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China, caij1@163.com.
  • About author:Shaogang Lin, Master. Shaogang Lin and Shuifen Ye contributed equally to this work.

PDF

2486

摘要：

给Wistar大鼠灌胃0.180g/mL的补肾中药，淫羊藿、菟丝子、肉苁蓉，0.04 mg/mL的单胺氧化酶B抑制剂司来吉兰或蒸馏水连续14 d，制备含药血清或空白血清。用15%各含药血清培养液培养对数生长期的MES23.5多巴胺能神经细胞24h后，加入含100μM H2O2培养液培养3h。MTT结果显示，淫羊藿、菟丝子、肉苁蓉及司来吉兰含药血清均能提高H2O2损伤的MES23.5细胞的存活率。流式细胞仪检测结果显示，淫羊藿、菟丝子、肉苁蓉及司来吉兰含药血清均能抑制H2O2损伤的MES23.5细胞凋亡相关蛋白FasL和Caspase-3的表达，促进抑凋亡蛋白Bcl-2的表达，而对Fas的表达无明显影响。ELISA结果显示，肉苁蓉和淫羊藿含药血清可提高H2O2损伤的MES23.5细胞神经生长因子、脑源性神经营养因子、胶质细胞源性生长因子的表达，而菟丝子含药血清仅能提高细胞脑源性神经营养因子的表达；司来吉兰含药血清对上述神经营养因子的表达均无明显促进作用。作者认为，这项研究结果证实了补肾中药可调节MES23.5细胞凋亡因子及神经营养因子的表达，产生保护神经细胞的效应。

关键词: 神经再生, 中医药, 含药血清, MES23.5多巴胺能神经细胞, 神经营养因子, 细胞凋亡因子, 帕金森, 神经保护, 基金资助文章

Abstract:

Wistar rats were intragastrically perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedii (Epimedium), Semen Cuscutae (Dodder Seed), or Herba Cistanches (Desertliving Cistanche), 0.04 mg/mL monoamine oxidase-B inhibitor selegiline, or distilled water for 14 consecutive days to prepare drug-containing serum or blank serum. MES23.5 cells in the logarithmic phase were cultured in media supplemented with 15% drug-containing serum for 24 hours, followed by incubation in culture solution containing 100 μmol/L H2O2 for 3 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry results showed that all drug-containing serums improved the survival rate of H2O2-injured MES23.5 cells, inhibited pro-apoptotic FasL and caspase-3 expression, promoted anti-apoptotic Bcl-2 expression. However, drug-containing serums had little influence on Fas expression in H2O2-injured MES23.5 cells. Enzyme-linked immunosorbent assay results showed that serum containing Herba Cistanches or Herba Epimedii increased the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in injured MES23.5 cells; serum containing Semen Cuscutae only increased brain-derived neurotrophic factor expres-sion; while expression of the above neurotrophic factors remained the same in cells treated with serum containing selegiline. These findings indicate that Chinese medicines used to tonify the kid-ney can protect nerve cells by regulating the expression of apoptosis-related factors and neurotrophic factors in MES23.5 cells.

Key words: neural regeneration, traditional Chinese medicine, drug-containing serum, MES23.5 dopaminergic nerve cells, neurotrophic factors, apoptosis factors, Parkinson’s disease, neuroprotection