中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (35): 3275-3283.doi: 10.3969/j.issn.1673-5374.2013.35.002

• 原著:退行性病与再生 • 上一篇    下一篇

帕金森病小鼠黑质致密区的硫氧还蛋白还原酶1蛋白表达下调

  

  • 收稿日期:2013-09-02 修回日期:2013-11-21 出版日期:2013-12-15 发布日期:2013-12-15

Downregulation of thioredoxin reductase 1 expression in the substantia nigra pars compacta of Parkinson’s disease mice

Zihua Liu1, Yuhong Jing2, 3, Jie Yin2, Jiying Mu2, Tingting Yao2, Liping Gao1, 3   

  1. 1 Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China
    2 Institute of Anatomy and Embryology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China
    3 Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, Gansu Province, China
  • Received:2013-09-02 Revised:2013-11-21 Online:2013-12-15 Published:2013-12-15
  • Contact: Liping Gao, Ph.D., Master’s supervisor, Professor, Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China; Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, Gansu Province, China, gaolp@lzu.edu.cn.
  • About author:Zihua Liu, Studying for master’s degree, working in Blood Transfusion Service, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China. Zihua Liu and Yuhong Jing contributed equally to this work.

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摘要：

鉴于神经细胞对氧化损伤的易感性及硫氧还蛋白还原酶1在中枢神经系统分布的广泛性及抗氧化特性，我们推测硫氧还蛋白还原酶1可能参与了帕金森病的发病。为此，实验采用1-甲基-4-苯基-1,2,3,6-四氢吡啶腹腔注射制备帕金森病小鼠模型。以实时RT-PCR、western blot、比色法检测显示，帕金森病模型小鼠中脑硫氧还蛋白还原酶1 mRNA和蛋白的表达、硫氧还蛋白还原酶的活性均明显降低。免疫组织化学染色显示，帕金森病模型小鼠中脑黑质致密区硫氧还蛋白还原酶1免疫阳性神经元的数量显著减少。结果说明，硫氧还蛋白还原酶1蛋白在帕金森病小鼠黑质致密区表达显著下调，可能与帕金森病的发病有关。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生 

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