中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (8): 845-850.doi: 10.4103/1673-5374.131601

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

西酞普兰能提高骨髓间充质干细胞向神经元样细胞的分化效力

  

  • 收稿日期:2014-03-14 出版日期:2014-04-25 发布日期:2014-04-25
  • 基金资助:

    伊朗德黑兰医科大学医学科技研究中心基金

Citalopram increases the differentiation efficacy of bone marrow mesenchymal stem cells into neuronal-like cells

Javad Verdi  1, 2, Seyed Abdolreza Mortazavi-Tabatabaei 1, 2, Shiva Sharif  2,  3, Hadi Verdi 2, Alireza Shoae-Hassani 1, 2   

  1. 1 Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2 Department of Stem Cells and Tissue Engineering, Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    3 Department of Tissue Engineering, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Received:2014-03-14 Online:2014-04-25 Published:2014-04-25
  • Contact: Alireza Shoae-Hassani , Italia St., Vesal Ave, Keshavarz Blv, Tohid Sq, Chamran Highway, Tehran 14185-615, Iran, cell.therapy@yahoo.com.
  • Supported by:

    This work was funded by the Research Center for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences, Tehran (TUMS), Tehran, Iran.

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摘要：

有研究显示，选择性5-羟色胺再吸收抑制剂类抗抑郁药可促进神经细胞增殖，提高神经可塑性。我们假设选择性5-羟色胺再吸收抑制剂西酞普兰对成体骨髓间充质干细胞的神经分化效力有促进作用。结果发现，西酞普兰干预后，神经诱导分化培养基中培养的骨髓间充质干细胞的神经元样细胞特征更为明显，诱导分化的骨髓间充质干细胞死亡率降低、增殖能力增强，骨髓间充质干细胞向神经元样细胞分化能力提高。说明西酞普兰通过促进诱导分化的骨髓间充质干细胞存活、增殖，维持其神经元样细胞特征，提高其向神经元细胞的分化效力。

关键词: 神经再生, 西酞普兰, 干细胞, 骨髓间充质干细胞, 存活, 增殖, 分化, 神经元

Abstract:

Several studies have demonstrated that selective serotonin reuptake inhibitor antidepressants can promote neuronal cell proliferation and enhance neuroplasticity both in vitro and in vivo. It is hypothesized that citalopram, a selective serotonin reuptake inhibitor, can promote the neuronal differentiation of adult bone marrow mesenchymal stem cells. Citalopram strongly enhanced neuronal characteristics of the cells derived from bone marrow mesenchymal stem cells. The rate of cell death was decreased in citalopram-treated bone marrow mesenchymal stem cells than in control cells in neurobasal medium. In addition, the cumulative population doubling level of the citalopram-treated cells was significantly increased compared to that of control cells. Also BrdU incorporation was elevated in citalopram-treated cells. These findings suggest that citalopram can improve the neuronal-like cell differentiation of bone marrow mesenchymal stem cells by increasing cell proliferation and survival while maintaining their neuronal characteristics.

Key words: nerve regeneration, citalopram, stem cells, bone marrow mesenchymal stem cells, survival, proliferation, differentiation, neurons, neural regeneration