中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (20): 1814-1819.doi: 10.4103/1673-5374.143427

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

脱细胞同种异体神经复合脑源性神经营养因子转染骨髓间充质干细胞：可更好的修复坐骨神经损伤效果优于单一方法

  

  • 收稿日期:2014-08-06 出版日期:2014-10-25 发布日期:2014-10-25

Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone

Yanru Zhang 1, 2, Hui Zhang 3, Gechen Zhang 3, Ka Ka 1, Wenhua Huang 2   

  1. 1 School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China
    2 Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China
    3 Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China
  • Received:2014-08-06 Online:2014-10-25 Published:2014-10-25
  • Contact: Wenhua Huang, Ph.D., Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515, Guangdong Province, China,jiaoxueban2010@126.com.

PDF

737

摘要：

实验设想用大鼠双侧坐骨神经制备化学去细胞同种异体神经，联合脑源性神经营养因子复合骨髓间充质干细胞桥接修复大鼠坐骨神经10 mm缺损，以期达到更好的修复效果。设脱细胞同种异体神经桥接组和脱细胞同种异体神经复合间充质干细胞组作对照。为此，发现桥接修复后8周，大鼠的坐骨神经指数、三头肌湿质量恢复率、髓鞘厚度、有髓神经纤维数量在3组中的变化为：脱细胞同种异体神经+脑源性神经营养因子+间充质干细胞组＞脱细胞同种异体神经+间充质干细胞组＞脱细胞同种异体神经组，说明化学脱细胞同种异体神经复合神经因子和间充质干细胞可促进坐骨神经缺损的修复，修复效果优于单独应用化学脱细胞同种异体神经或脱细胞同种异体神经复合间充质干细胞移植。

关键词: 神经再生, 周围神经再生, 周围神经损伤, 化学脱细胞同种异体神经, 脑源性神经营养因子, 骨髓间充质干细胞, 坐骨神经, 坐骨神经指数, 湿质量恢复率, 神经组织工程

Abstract:

In this study, we chemically extracted acellular nerve allografts from bilateral sciatic nerves, and repaired 10-mm sciatic nerve defects in rats using these grafts and brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells. Experiments were performed in three groups: the acellular nerve allograft bridging group, acellular nerve allograft + bone marrow mesenchymal stem cells group, and the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group. Results showed that at 8 weeks after bridging, sciatic functional index, triceps wet weight recovery rate, myelin thickness, and number of myelinated nerve fibers were significantly changed in the three groups. Variations were the largest in the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group compared with the other two groups. Experimental findings suggest that chemically extracted acellular nerve allograft combined nerve factor and mesenchymal stem cells can promote the restoration of sciatic nerve defects. The repair effect seen is better than the single application of acellular nerve allograft or acellular nerve allograft combined mesenchymal stem cell transplantation.

Key words: nerve regeneration, peripheral nerve regeneration, peripheral nerve injury, chemically extracted acellular nerve, brain-derived neurotrophic factor, bone marrow mesenchymal stem cells, nerve tissue engineering, neural regeneration