中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (22): 1979-1984.doi: 10.4103/1673-5374.145379

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

甲钴胺对小鼠坐骨神经夹伤修复的作用机

  

  • 收稿日期:2014-10-20 出版日期:2014-11-25 发布日期:2014-11-25

Restorative effect and mechanism of mecobalamin on sciatic nerve crush injury in mice

Lin Gan, Minquan Qian, Keqin Shi, Gang Chen, Yanglin Gu, Wei Du, Guoxing Zhu   

  1. Department of Orthopedics, Wuxi No. 2 People’s Hospital, Wuxi, Jiangsu Province, China
  • Received:2014-10-20 Online:2014-11-25 Published:2014-11-25
  • Contact: Wei Du, Department of Orthopedics, Wuxi No. 2 People’s Hospital, 68 Zhongshan Road, Wuxi 214002, Jiangsu Province, China, dujingjiang@sina.com. Guoxing Zhu, Department of Orthopedics, Wuxi No. 2 People’s Hospital, 68 Zhongshan Road, Wuxi 214002, Jiangsu Province, China, guoxing-zhu@163.com.
  • Supported by:

    This study was supported by Nanjing Medical University Technology Development Fund of China (General Program), No. 2013NJMU182.

摘要:

甲钴胺是一种含金属元素钴的维生素B12,是与神经系统功能相关的重要因子之一,在临床上常用于促进周围神经功能的恢复,但至目前人们尚不清楚其作用的分子机制。为此,实验给予坐骨神经夹伤模型小鼠腹腔注射甲钴胺130,65 μg/kg,并设生理盐水组作对照。足迹实验、组织形态学检查、real-time PCR结果显示,与生理盐水组相比,甲钴胺腹腔注射可改善坐骨神经夹伤模型小鼠坐骨神经功能,增加有髓神经纤维的髓鞘厚度和靶肌细胞横截面积,上调小鼠损伤侧神经组织生长相关蛋白43及脊髓L4-L6节段背根神经节中神经营养因子、碱性成纤维细胞生长因子和睫状神经生长因子mRNA的表达,实验结果阐释了甲钴胺促近坐骨神经损伤修复的分子机制。

关键词: 神经再生, 周围神经损伤, 甲钴胺, 坐骨神经, 神经修复, 神经营养因子, 神经保护, 维生素B12, 分子机制, 基因表达

Abstract:

Mecobalamin, a form of vitamin B12 containing a central metal element (cobalt), is one of the most important mediators of nervous system function. In the clinic, it is often used to accelerate recovery of peripheral nerves, but its molecular mechanism remains unclear. In the present study, we performed sciatic nerve crush injury in mice, followed by daily intraperitoneal administration of mecobalamin (65 μg/kg or 130 μg/kg) or saline (negative control). Walking track analysis, histomorphological examination, and quantitative real-time PCR showed that mecobalamin significantly improved functional recovery of the sciatic nerve, thickened the myelin sheath in myelinated nerve fibers, and increased the cross-sectional area of target muscle cells. Furthermore, mecobalamin upregulated mRNA expression of growth associated protein 43 in nerve tissue ipsilateral to the injury, and of neurotrophic factors (nerve growth factor, brain-derived nerve growth factor and ciliary neurotrophic factor) in the L4–6 dorsal root ganglia. Our findings indicate that the molecular mechanism underlying the therapeutic effect of mecobalamin after sciatic nerve injury involves the upregulation of multiple neurotrophic factor genes.

Key words: nerve regeneration, peripheral nerve injury, mecobalamin, sciatic nerve, nerve repair, neurotrophic factor, neuroprotective effect, vitamin B12, molecular mechanism, gene expression, neural regeneration