中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (22): 1995-2001.doi: 10.4103/1673-5374.145383

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

中枢神经系统炎症表达及定位中的CD93与GIPC

  

  • 收稿日期:2014-09-05 出版日期:2014-11-25 发布日期:2014-11-25
  • 基金资助:

    国家自然科学基金项目（31170766），南通市社会事业科技创新与示范计划项目（HS2012032），南通大学2012年校级自然科学类科研基金前期预研项目（12ZY020）

CD93 and GIPC expression and localization during central nervous system inflammation

Chun Liu 1, Zhichao Cui 2, Shengjie Wang 1, Dongmei Zhang 2   

  1. 1 Experimental Animal Center, Key Laboratory of Inflammation and Molecular Drug Targets of Jiangsu Province, Nantong University, Nantong, Jiangsu Province, China
    2 Department of Pathogen Biology, Medical School of Nantong University, Nantong, Jiangsu Province, China
  • Received:2014-09-05 Online:2014-11-25 Published:2014-11-25
  • Contact: Dongmei Zhang, M.D., Department of Pathogen Biology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China, zdm@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31170766; the Nantong Municipal Social Undertakings Technological Innovation and Demonstration Project Foundation, No. HS2012032; the Natural Science Pre-research Project Foundation of Nantong University in 2012, No. 12ZY020.

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摘要：

以往研究发现CD93与GIPC可相互作用调节免疫细胞吞噬过程，但CD93和GIPC在中枢神经系统炎症中的表达与定位未见报道。鉴于此，实验利用脂多糖注射大鼠侧脑室构建大脑炎症模型。以Western Blot检测显示，CD93在致炎大鼠脑的表达随时间变化呈先增加而后递减趋势，而GIPC的表达无明显变化。免疫组织化学检测显示，CD93在大鼠大脑皮质广泛分布，主要表达于胞膜，脂多糖刺激后CD93表达先增加而后下降，且有明显胞浆及胞核染色。免疫荧光双标检测显示，在正常大鼠的大脑皮质中，CD93和GIPC广泛表达于静息状态小胶质细胞和神经元中，CD93主要表达于小胶质细胞和神经元的胞膜上，而GIPC在胞膜和胞浆均有分布。建模后9 h，大脑皮质小胶质细胞胞膜CD93阳性信号显著减弱，出现胞浆的聚集转位，而GIPC在神经元及小胶质细胞的定位无明显变化。结果首次描述了CD93参与中枢神经系统炎症的病理生理过程。

关键词: 中枢神经系统, 大脑炎症模型, CD93, GIPC, 神经元, 小胶质细胞, 表达及定位, 脂多糖, 侧脑室注射, 大鼠, 诱导型一氧化氮合酶, 国家自然科学基金

Abstract:

CD93 and GAIP-interacting protein, C termius (GIPC) have been shown to interactively alter phagocytic processes of immune cells. CD93 and GIPC expression and localization during central nervous system inflammation have not yet been reported. In this study, we established a rat model of brain inflammation by lipopolysaccharide injection to the lateral ventricle. In the brain of rats with inflammation, western blots showed increased CD93 expression that decreased over time. GIPC expression was unaltered. Immunohistochemistry demonstrated extensive distribution of CD93 expression mainly in cell membranes in the cerebral cortex. After lipopolysaccharide stimulation, CD93 expression increased and then reduced, with distinct staining in the cytoplasm and nucleus. Double immunofluorescence staining in cerebral cortex of normal rats showed that CD93 and GIPC widely expressed in resting microglia and neurons. CD93 was mainly expressed in microglial and neuronal cell membranes, while GIPC was expressed in both cell membrane and cytoplasm. In the cerebral cortex at 9 hours after model establishment, CD93-immunoreactive signal diminished in microglial membrane, with cytoplasmic translocation and aggregation detected. GIPC localization was unaltered in neurons and microglia. These results are the first to demonstrate CD93 participation in pathophysiological processes of central nervous system inflammation.

Key words: nerve regeneration, central nervous system, brain inflammation model, CD93, GIPC, neurons, microglia, expression and localization, lipopolysaccharide, intracerebroventricular injection, rats, inducible nitric oxide synthase, NSFC grants, neural regeneration