中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (1): 104-111.doi: 10.4103/1673-5374.150715

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

可降解甲壳质生物套管联合骨髓间充质干细胞移植修复脊髓损伤：可减少胶质瘢痕和空洞形成

  

  • 收稿日期:2014-12-10 出版日期:2015-01-15 发布日期:2015-01-15
  • 基金资助:

    科技部973计划(No. 2014CB542201)；教育部创新团队(IRT1201)；国家自然科学基金 (No. 31271284、31171150、81171146, 30971526, 31100860，31040043), 教育部新世纪优秀人才（BMU20110270) 、北京市自然基金（Beijing Natural Science Foundation NO:7142164）

Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by  reducing glial scar and cavity formation

Feng Xue 1, #, Er-jun Wu 2, #, Pei-xun Zhang 1, #, Li-ya A 1, Yu-hui Kou 1, *, Xiao-feng Yin 1, *, Na Han 3, *   

  1. 1 Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China
    2 Graduate School of Shanxi Medical University, Taiyuan, Shanxi Province, China
    3 Central Laboratory, Peking University People’s Hospital, Beijing, China
  • Received:2014-12-10 Online:2015-01-15 Published:2015-01-15
  • Contact: Na Han, Ph.D., 876804705@qq.com. Yu-hui Kou, Ph.D., kouyuhui@163.com. Xiao-feng Yin, Ph.D., xiaofengyin@bjmu.edu.cn.
  • Supported by:

    This study was supported by grants from the National Program on Key Basic Research Project of China (973 Program), No. 2014CB542201; Program for Innovative Research Team in University of Ministry of Education of China, No. IRT1201; the National Natural Science Foundation of China, No. 31271284, 31171150, 81171146, 30971526, 31100860, 31040043; Program for New Century Excellent Talents in University of Ministry of Education of China, No. BMU20110270; the Natural Science Foundation of Beijing of China, No. 7142164.

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摘要：

为骨髓间充质干细胞在脊髓内存活分化的局部微环境，提高对脊髓损伤的修复效果，实验将可降解甲壳质生物套管经过创新改进后联合骨髓间充质干细胞，验证其修复大鼠右侧脊髓半切损伤的效果。免疫组织化学染色检测发现，生物套管套接修复脊髓半切损伤可以减少胶质瘢痕和脊髓空洞形成。逆行示踪检测显示，干预14周时脊髓损伤位点远近端均有较多的神经纤维生长通过。骨髓间充质干细胞体内移植后存活增殖情况良好，干预8周时可分化为具有许旺细胞表型S100和胶质细胞表型胶质纤维酸性蛋白的阳性细胞。说明生物套管联合骨髓间充质干细胞移植修复脊髓半切损伤，有效阻止了瘢痕组织的侵入，套管所构建的局部微环境为骨髓间充质干细胞在脊髓中的增殖、迁移和分化创造了条件。

关键词: 神经再生, 脊髓损伤, 脊髓半切, 生物套管, 骨髓间充质干细胞, 干细胞, 透射电镜, 细胞移植, 神经元, 神经纤维, 国家自然科学基金

Abstract:

We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvironment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury.

Key words: nerve regeneration, spinal cord injury, spinal cord hemisection, biological conduit, bone marrow mesenchymal stem cells, stem cells, transmission electron microscope, cell transplantation, neurons, nerve fibers, NSFC grants, neural regeneration