中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (10): 1678-1684.doi: 10.4103/1673-5374.193250

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

甲基泼尼松龙与罗格列酮联合干预可以更好的促进脊髓损伤后神经功能恢复

  

  • 收稿日期:2016-09-07 出版日期:2016-10-31 发布日期:2016-10-31

Combination of methylprednisolone and rosiglitazone promotes recovery of neurological function afer spinal cord injury

Xi-gong Li, Xiang-jin Lin, Jun-hua Du*, San-zhong Xu, Xian-feng Lou, Zhong Chen   

  1. Department of Orthopedic Surgery, First Afliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
  • Received:2016-09-07 Online:2016-10-31 Published:2016-10-31
  • Contact: Jun-hua Du, M.D., zyorthopaedic@yahoo.com.cn.

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摘要：

甲基泼尼松龙具有抗炎抗氧化的特性，罗格列酮则通过激活脊髓组织中的过氧化物酶体增殖激活型受体γ具有抗炎和抗氧化作用，两者临床上已被用于脊髓继发性损伤的早期药物治疗。由于脊髓损伤后炎症过程的复杂性及多样性，采用单一药物干预难以完全抑制损伤后的炎症过程。我们假设甲基泼尼松龙与罗格列酮联合干预可以更好的促进脊髓继发性损伤后神经功能恢复。实验设计为大鼠脊髓损伤后1 h腹腔注射甲基泼尼松龙30 mg/kg，脊髓损伤后24，48 h再腹腔注射基泼尼松龙15 mg/kg，脊髓损伤后1 h注射罗格列酮2 mg/kg，以后每12 h注射罗格列酮1次，连续7 d。结果发现甲基泼尼松龙与罗格列酮联合干预较两者单独应用能显著减轻脊髓继发性损伤大鼠脊髓组织的炎症反应和细胞凋亡，改善大鼠的运动功能。说明甲基泼尼松龙与罗格列酮联合干预可以更好的促进脊髓继发性损伤损伤后的神经功能恢复，效果优于两者单独应用。 

orcid: 0000-0002-0786-3410 (Jun-hua Du)

关键词: 神经再生, 脊髓损伤, 甲基泼尼松龙, 罗格列酮, 炎症, 药物疗法, 抗炎剂, PPARγ激动剂, 功能恢复, 病理学, 动物, 大鼠

Abstract: Methylprednisolone exhibits anti-in?ammatory antioxidant properties, and rosiglitazone acts as an anti-in?ammatory and antioxidant by activating peroxisome proliferator-activated receptor-γ in the spinal cord. Methylprednisolone and rosiglitazone have been clinically used during the early stages of secondary spinal cord injury. Because of the complexity and diversity of the in?ammatory process afer spinal cord injury, a single drug cannot completely inhibit in?ammation. Terefore, we assumed that a combination of methylprednisolone and rosiglitazone might promote recovery of neurological function afer secondary spinal cord injury. In this study, rats were intraperitoneally injected with methylprednisolone (30 mg/kg) and rosiglitazone (2 mg/kg) at 1 hour afer injury, and methylprednisolone (15 mg/kg) at 24 and 48 hours afer injury. Rosiglitazone was then administered once every 12 hours for 7 consecutive days. Our results demonstrated that a combined treatment with methylprednisolone and rosiglitazone had a more pronounced e?ect on attenuation of in?ammation and cell apoptosis, as well as increased functional recovery, compared with either single treatment alone, indicating that a combination better promoted recovery of neurological function afer injury.

Key words: nerve regeneration, spinal cord injury, methylprednisolone, rosiglitazone, in?ammation, drug therapy, anti-in?ammatory agents, functional recovery, neural regeneration