中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (8): 1287-1293.doi: 10.4103/1673-5374.213548

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

银杏叶提取物促进染毒后海马神经元的再生

  

  • 收稿日期:2017-07-25 出版日期:2017-08-15 发布日期:2017-08-15
  • 基金资助:

    广东省自然科学基金（2014A030310455）; 广州市Pearl River S&T Nova项目基金（201710010002）

Extract of Ginkgo biloba promotes neuronal regeneration in the hippocampus after exposure to acrylamide

Wei-ling Huang1, Yu-xin Ma1, Yu-bao Fan1, Sheng-min Lai1, Hong-qing Liu1, Jing Liu1, Li Luo1, Guo-ying Li1, Su-min Tian2   

  1. 1 Department of Anatomy, School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China;                                          
    2 Department of Physiology, School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China
  • Received:2017-07-25 Online:2017-08-15 Published:2017-08-15
  • Contact: Su-min Tian, M.D.,guangyaosumin@sina.com.
  • Supported by:

    This study was supported by the Natural Science Foundation of Guangdong Province of China, No. 2014A030310455; the Pearl River S&T Nova Program Foundation of Guangzhou City of China, No. 201710010002.

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摘要：

既往研究表明银杏叶提取物对神经元损伤具有明显改善作用，其相关机制研究集中在其抗氧化作用方面，关于银杏叶提取物对神经元的神经毒性损伤是否有保护作用的研究较少。为此，试验设计连续4周给小鼠丙烯酰胺灌胃建立染毒小鼠模型，然后用30，60，120 mg/kg银杏叶提取物灌胃模型小鼠发现银杏叶提取物各剂量均能有效缓解染毒模型小鼠的异常步态，并上调海马脑源性神经营养因子、神经元标记物生长相关蛋白43及神经元标记物双皮质素抗体蛋白的表达，并伴随生长相关蛋白43蛋白及双皮质素抗体免疫阳性细胞的增多。说明银杏叶提取物能减轻丙烯酰胺所致的神经元毒性反应，促进了染毒小鼠海马神经元再生。

orcid:0000-0002-2707-1402(Su-min Tian)

关键词: 神经再生, 脑损伤, 银杏叶提取物, 丙烯酰胺, 双皮质素抗体, 脑源性神经营养因子, 生长相关蛋白43, 神经元, 损伤, 海马, 小鼠

Abstract:

Previous studies have demonstrated a neuroprotective effect of extract of Ginkgo biloba against neuronal damage, but have mainly focused on antioxidation of extract of Ginkgo biloba. To date, limited studies have determined whether extrasct of Ginkgo biloba has a protective effect on neuronal damage. In the present study, acrylamide and 30, 60, and 120 mg/kg extract of Ginkgo biloba were administered for 4 weeks by gavage to establish mouse models. Our results showed that 30, 60, and 120 mg/kg extract of Ginkgo biloba effectively alleviated the abnormal gait of poisoned mice, and up-regulated protein expression levels of doublecortin (DCX), brain-derived neurotrophic factor, and growth associated protein-43 (GAP-43) in the hippocampus. Simultaneously, DCX- and GAP-43-immunoreactive cells increased. These findings suggest that extract of Ginkgo biloba can mitigate neurotoxicity induced by acrylamide, and thereby promote neuronal regeneration in the hippocampus of acrylamide-treated mice.

Key words: nerve regeneration, brain injury, extrat of Ginkgo biloba, acrylamide, doublecortin, brain-derived neurotrophic factor, growth associated protein-43, neurons, damage, hippocampus, mice, neural regeneration