中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (6): 1013-1018.doi: 10.4103/1673-5374.233444

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

穴位埋线可增加脑卒中后痉挛大鼠大脑中谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1的表达

  

  • 收稿日期:2018-05-15 出版日期:2018-06-15 发布日期:2018-06-15

Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke

Rui-Qing Li1, Ming-Yue Wan2, Jing Shi2, Hui-Ling Wang2, Fei-Lai Liu1, Cheng-Mei Liu1, Jin Huang2, Ren-Chao Liu2, Le Ma3, Xiao-Dong Feng1   

  1. 1 Rehabilitation Center, First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
    2 Major in Rehabilitation Medicine and Physiotherapy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
    3 Department of Oncology, Third People’s Hospital of Luoyang, Luoyang, Henan Province, China
  • Received:2018-05-15 Online:2018-06-15 Published:2018-06-15
  • Contact: Xiao-Dong Feng, M.D., Ph.D.,13733165164@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 14202225, &. 81574042; the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China, No. 2000202; a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China, No. JDZX2015314.

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摘要：

很多研究已显示穴位埋线可改善卒中大鼠的痉挛状态，但尚未发现其作用机制。试验设计建立大脑中动脉闭塞脑卒中大鼠模型，3d后在大椎、脊中、后会、关元和中脘穴植入可吸收外科缝线。以Zea-Longa神经缺陷评分评估神经功能，以改良Ashworth量表评价肌肉张力，以TTC染色测量脑梗死体积，以免疫组化染色谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1的免疫阳性反应，以Western blot分析谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1的表达，以反转录PCR检测谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1mRNA的表达。结果显示，经穴位埋线治疗后，脑卒中后痉挛大鼠神经功能明显改善，肌张力下降，脑梗死体积缩小，损伤侧(左侧)脑组织中谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1蛋白及mRNA的表达增加，数据结果提示穴位埋线可通过增加谷氨酸天冬氨酸转运体和神经胶质谷氨酸转运体1的表达，改善脑卒中后的痉挛。

orcid:0000-0002-3806-7388(Xiao-Dong Feng)

关键词: 卒中, 大椎, 脊中, 后会, 关元, 中脘, 穴位埋线, 肢体痉挛, 谷氨酸转运蛋白, 神经再生

Abstract:

Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats. However, the underlying mechanisms are poorly understood. In this study, we used the rat middle cerebral artery occlusion model of stroke. Three days after surgery, absorbable surgical catgut sutures were implanted at Dazhui (GV14), Jizhong (GV6), Houhui, Guanyuan (CV4) and Zhongwan (CV12). The Zea Longa score was used to assess neurological function. The Modified Ashworth Scale was used to evaluate muscle tension. The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume. Immunohistochemical staining was performed for glutamate aspartate transporter (GLAST) and glial glutamate transporter-1 (GLT-1) expression. Western blot assay was used to analyze the expression of GLAST and GLT-1. Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1 mRNAs. After catgut implantation at the acupoints, neurological function was substantially improved, muscle tension was decreased, and infarct volume was reduced in rats with spasticity after stroke. Furthermore, the expression of GLAST and GLT-1 mRNAs was increased on the injured (left) side. Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke, likely by increasing the expression of GLAST and GLT-1.

Key words: nerve regeneration, stroke, Dazhui (GV14), Jizhong (GV6), Houhui, Guanyuan (CV4), Zhongwan (CV12), catgut implantation at acupoints, limb spasm, glutamate transporter, neural regeneration