中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (8): 1425-1432.doi: 10.4103/1673-5374.235298

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺“百会”透“曲鬓”减轻脑出血后神经功能损伤

  

  • 收稿日期:2018-06-22 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:

    中国国家自然科学基金项目（81473764, 81273824），黑龙江省自然科学基金项目重点项目（ZD201204），中国教育部博士基金项目（20102327110003）

Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage

Xiao-Ying Liu1, Xiao-Hong Dai2, Wei Zou2, 3, Xue-Ping Yu2, Wei Teng2, Ying Wang4, Wei-Wei Yu2, Hui-Hui Ma2, Qiu-Xin Chen2,Peng Liu1, Rui-Qiao Guan1, Shan-Shan Dong1   

  1. 1 Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    2 The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    3 Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine of Heilongjiang University of Chinese Medicine, Harbin,Heilongjiang Province, China
    4 Department of Pharmacology of Dali University, Dali, Yunnan Province, China
  • Received:2018-06-22 Online:2018-08-15 Published:2018-08-15
  • Contact: Wei Zou, Ph.D. or Xue-Ping Yu,Ph.D., kuangzou1965@163.com or hzyyxp82@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81473764, 81273824; the Key Project of Natural Science Foundation of Heilongjiang Province of China, No. ZD201204; the Doctoral Fund Program of Ministry of Education of China, No. 20102327110003.

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摘要：

 

炎症在脑出血引起的神经缺损中发挥重要作用。抑制巨噬细胞诱导的C型凝集素受体/脾酪氨酸激酶信号通路修复脑损伤是治疗脑出血的潜在方法。实验拟探索针刺“百会”透“曲鬓”是否通过该途径对脑出血产生治疗作用。使用自体血液输注尾状核建立脑出血大鼠模型，针刺“百会”透“曲鬓”30min，每12h一次，进行3次治疗，并以腹腔注射34.62 mg/kg白皮杉醇（脾酪氨酸激酶抑制剂）治疗作为对照。以改良的神经严重度评分评价神经功能，以电子天平称量脑水含量，以免疫组织化学和Western blot检测损伤脑组织中Mincle，Syk和CARD9的免疫阳性反应及蛋白表达水平，以实时PCR测量损伤脑组织中白细胞介素1βmRNA的水平，以苏木精-伊红染色观察损伤脑组织病理学改变。发现针刺“百会”透“曲鬓”穴位，可明显改善脑出血大鼠的神经功能、脑含水量，抑制损伤脑组织中Mincle，Syk和CARD9的免疫阳性反应及表达水平和白细胞介素1βmRNA的水平，且效果与白皮杉醇接近。说明“百会”透“曲鬓”穴位针刺通过抑制巨噬细胞诱导的C型凝集素受体/脾酪氨酸激酶信号通路，改善了脑出血的神经功能损伤。

orcid:0000-0003-4560-1582(Wei Zou)
        0000-0002-9929-4883(Xue-Ping Yu)

关键词: 脑出血, 针刺, 百会, 曲鬓, 炎症, 巨噬细胞诱导的C型凝集素受体/脾酪氨酸激酶信号通路, 神经保护, 神经再生

Abstract:

Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase (Mincle/Syk) signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui (DU20) to Qubin (GB7) is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autologous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours,for a total of three times. Piceatannol (34.62 mg/kg), a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1β mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1β. Moreover, this effect was similar to piceatannol. These findings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.

Key words: nerve regeneration, intracerebral hemorrhage, acupuncture, Baihui (DU20), Qubin (GB7), inflammation, Mincle/Syk signaling pathway, nerve protection, neural regeneration