中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (11): 2310-2315.doi: 10.4103/1673-5374.310701

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

胚胎和产后早期大鼠肠神经嵴前体细胞表型和功能的比较研究

  

  • 出版日期:2021-11-15 发布日期:2021-04-13
  • 基金资助:

    国家自然科学基金项目（82071692，81741096，81770513，81270435）；陕西省青年科技项目（2019KJXX-044）；陕西省自然科学基金项目（2020JM-407）；西安交通大学第二附属医院基金项目（第RC（XM）201703号）

Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages

Dong-Hao Tian, Chuan-Hui Qin, Wen-Yao Xu, Wei-Kang Pan, Yu-Ying Zhao, Bai-Jun Zheng, Xin-Lin Chen, Yong Liu, Ya Gao, Hui Yu   

  1. 1Department of Pediatric Surgery, the Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China; 2Department of Anorectal, Suizhou Central Hospital, Hubei University of Medicine, Suizhou, Hubei Province, China; 3Institute of Neurobiology, Environment and Genes Related to Diseases Key Laboratory of Chinese Ministry of Education, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Online:2021-11-15 Published:2021-04-13
  • Contact: Ya Gao, MD, PhD, ygao@mail.xjtu.edu.cn; Hui Yu, MD, PhD, yuhui831022@gmail.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 82071692 (to HY), 81741096 (to HY), 81770513 (to YG), 81270435 (to WKP); Shaanxi Youth Science and Technology Project of China, No. 2019KJXX-044 (to HY); the National Natural Science Foundation of Shaanxi Province of China, No. 2020JM-407 (to HY); and Fund Project of the Second Affiliated Hospital, Xi’an Jiaotong University No. RC(XM)201703 (to HY). 

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摘要：

作者既往研究显示，随着培养时间的延长，肠神经嵴前体细胞的生长特征会发生变化，且体外培养的产后肠神经嵴前体细胞的增殖、迁移和神经分化潜能明显降低。然而尚无研究比较胚胎和产后肠神经嵴前体细胞的差异。实验分别从大鼠胚胎（E14.5d）和新生（出生后2d）大鼠肠中分离出胚胎和产后肠神经嵴前体细胞。发现（1）与产后肠神经嵴前体细胞相比，胚胎肠神经嵴前体细胞衍生的神经球最大横截面更大，迁移能力更强，凋亡明显减少，但两者的分化能力接近；（2）在移植治疗先天性巨结肠大鼠模型中，移植的胚胎肠神经嵴前体细胞和产后肠神经嵴前体细胞均能存活。但与产后肠神经嵴前体细胞相比，移植的胚胎肠神经嵴前体细胞的大鼠肠神经丛神经节细胞数量更多，肠段腔内压力变化更为显著；（3）表明与产后肠神经嵴前体细胞相比，胚胎肠神经嵴前体细胞具有更好的存活、增殖和迁移能力，是更适宜的治疗先天性巨结肠种子细胞来源。实验于2016年3月3日经西安交通大学第二附属医院伦理委员会批准，批准号2016086。

https://orcid.org/0000-0002-6729-6374 (Ya Gao); https://orcid.org/0000-0002-4617-7640 (Hui Yu) 

关键词:

干细胞, 肠神经嵴前体细胞, 先天性巨结肠, 细胞表型, 细胞移植, 肠神经系统

Abstract: In our previous study, we showed that with increasing time in culture, the growth characteristics of enteric neural crest-derived cells (ENCCs) change, and that the proliferation, migration and neural differentiation potential of these cells in vitro notably diminish. However, there are no studies on the developmental differences in these characteristics between fetal and early-postnatal stages in vitro or in vivo. In this study, we isolated fetal (embryonic day 14.5) and postnatal (postnatal day 2) ENCCs from the intestines of rats. Fetal ENCCs had greater maximum cross-sectional area of the neurospheres, stronger migration ability, and reduced apoptosis, compared with postnatal ENCCs. However, fetal and postnatal ENCCs had a similar differentiation ability. Fetal and postnatal ENCCs both survived after transplant into a rat model of Hirschsprung’s disease. In these rats with Hirschsprung’s disease, the number of ganglionic cells in the myenteric plexus was higher and the distal intestinal pressure change was greater in animals treated with fetal ENCCs compared with those treated with postnatal ENCCs. These findings suggest that, compared with postnatal ENCCs, fetal ENCCs exhibit higher survival and proliferation and migration abilities, and are therefore a more appropriate seed cell for the treatment of Hirschsprung’s disease. This study was approved by the Animal Ethics Committee of the Second Affiliated Hospital of Xi’an Jiaotong University (approval No. 2016086) on March 3, 2016.

Key words:  cell transplantation, enteric nervous system, enteric neural crest-derived cells, Hirschsprung’s disease, stem cells

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