中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (10): 2247-2252.doi: 10.4103/1673-5374.336876

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

脑缺血再灌注急性期周细胞的时程变化

  

  • 出版日期:2022-10-15 发布日期:2022-03-16
  • 基金资助:
    中国中医科学院创新基金项目(CI2021A03407);中国国家自然科学基金项目(81973789)

Temporal alterations in pericytes at the acute phase of ischemia/reperfusion in the mouse brain

Shuang Zhang1, Xue-Jing Liao1, Jia Wang2, Yi Shen2, Han-Fen Shi1, Yan Zou3, Chong-Yang Ma4, Xue-Qian Wang1, Qing-Guo Wang1, Xu Wang1, Ming-Yang Xu1, Fa-Feng Cheng1, Wan-Zhu Bai2   

  1. 1Beijing Key Laboratory, School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China; 2Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China; 3Shineway Pharmaceutical Group Ltd., Shijiazhuang, Hebei Province, China; 4School of Traditional Chinese Medicine, Capital Medical University, Beijing, China
  • Online:2022-10-15 Published:2022-03-16
  • Contact: Fa-Feng Cheng, PhD, fafengcheng@gmail.com; Wan-Zhu Bai, PhD, wanzhubaisy@hotmail.com.
  • Supported by:
    This study was financially supported by the China Academy of Chinese Medical Sciences Innovation Fund, No. CI2021A03407 (to WZB) and the National Natural Science Foundation of China, No. 81973789 (to FFC).

摘要:

周细胞作为微血管周围的壁细胞,在微循环的调节中起着至关重要的作用;然而这些细胞对缺血性卒中的反应尚不清楚。为了解缺血再灌注后周细胞的时程变化,实验建立了脑缺血1h再灌注2,12,24h模型。结果发现,随着再灌注时间的延长,脑血流量逐渐减少,而梗死体积逐渐增加。同时发现再灌注24h内,梗死区中的周细胞收缩且作用于血管内皮。这可能是导致微循环再灌注不完全,并在急性期逐渐恶化的原因。这一结果将为理解周细胞在阻断缺血组织再灌注中的作用提供帮助。

https://orcid.org/0000-0001-6285-7788 (Wan-Zhu Bai)

关键词: 周细胞, 急性缺血性卒中, 无复流现象, 微循环, 血小板衍生生长因子受体β, α-平滑肌肌动蛋白, 血管内皮细胞, 血小板内皮细胞粘附分子1, 脑血流量

Abstract: Pericytes, as the mural cells surrounding the microvasculature, play a critical role in the regulation of microcirculation; however, how these cells respond to ischemic stroke remains unclear. To determine the temporal alterations in pericytes after ischemia/reperfusion, we used the 1-hour middle cerebral artery occlusion model, which was examined at 2, 12, and 24 hours after reperfusion. Our results showed that in the reperfused regions, the cerebral blood flow decreased and the infarct volume increased with time. Furthermore, the pericytes in the infarct regions contracted and acted on the vascular endothelial cells within 24 hours after reperfusion. These effects may result in incomplete microcirculation reperfusion and a gradual worsening trend with time in the acute phase. These findings provide strong evidence for explaining the “no-reflow” phenomenon that occurs after recanalization in clinical practice.

Key words: acute ischemic stroke, alpha-smooth muscle, cerebral blood flow, microcirculation, no-reflow phenomenon, pericytes, platelet endothelial cell adhesion molecule-1, platelet-derived growth factor receptor beta, vascular endothelial cells