中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (7): 3187-3193.doi: 10.4103/NRR.NRR-D-24-01505

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

储存的存活的末梢神经异体移植物经聚乙二醇融合修复损伤周围神经

  

  • 出版日期:2026-07-15 发布日期:2026-03-31

Successful polyethylene glycol fusion repair using stored viable peripheral nerve allografts in Sprague–Dawley and Lewis rats

Liwen Zhou1, Cathy Z. Yang1, Alexander M. Schafer1, Alexa N. Olivarez1, Arjun Agarwal1, Guhan Periyasamy1, Karthik Venkudusamy1, Yessenia Montoya1, Varun Gokhale1, Rhea Sood1, Henry Garcia1, Jared S. Bushman2, George D. Bittner1, *   

  1. 1Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA;  2School of Pharmacy, University of Wyoming, Laramie, WY, USA
  • Online:2026-07-15 Published:2026-03-31
  • Contact: George D. Bittner, PhD, bittner@austin.utexas.edu.
  • Supported by:
    This study was supported by the National Institutes of Health (NIH) R01-NS128086 grant (to GDB and JSB) and Lone Star Paralysis Foundation (to GDB).

摘要:

以前研究成功使用聚乙二醇融合修复大鼠节段性末梢神经损伤,使用的是能传导动作电位的新鲜、存活的末梢神经异体移植物。由于聚乙二醇与存活的末梢神经异体移植物融合的临床应用需要移植前供体组织的储存,因此开发了一种将大鼠坐骨神经作为存活的末梢神经异体移植物在体外储存的方案,可将许多轴突保存长达 5 天。目前的研究评估了这些储存的存活的末梢神经异体移植物在体内的使用情况。实验假设,储存的存活的末梢神经异体移植物具有存活的轴突,可通过聚乙二醇融合成功实现节段性末梢神经损伤的体内修复。根据坐骨神经功能指数的评估,聚乙二醇-融合的存活的末梢神经异体移植物在修复后 8 周内如果能明显改善运动功能的恢复,则被视为成功修复。许多成功聚乙二醇融合存活的末梢神经异体移植物的Sprague-Dawley和Lewis大鼠从术后5周开始坐骨神经功能明显改善。在成功进行聚乙二醇融合的效率和程度方面,储存的存活的末梢神经异体移植物与新鲜收获的存活的末梢神经异体移植物没有明显差异。相比之下,未融合阴性对照存活的末梢神经异体移植物的大鼠在术后 8 周内没有任何恢复。体内复合动作电位和轴突形态学评估结果也验证了储存的存活神经与聚乙二醇同种异体移植修复损伤周围神经的效果。这些结果表明,存活的末梢神经异体移植物可以储存起来,随后用于成功的损伤节段性末梢神经聚乙二醇融合修复。


https://orcid.org/0000-0002-5610-6264 (George D. Bittner)

关键词: 轴突形态计量学, 轴切术, 周围神经损伤, 周围神经修复, 聚乙二醇融合, 坐骨神经消融, 组织储存溶液

Abstract:

We have previously shown the success of polyethylene glycol fusion repair of segmental-loss peripheral nerve injuries in rats using freshly harvested, viable peripheral nerve allografts that can conduct action potentials. Because clinical application of polyethylene glycol fusion with viable peripheral nerve allografts demands pre-transplant donor tissue storage, we developed a protocol for ex vivo storage of rat sciatic nerves as viable peripheral nerve allografts, preserving many axons for up to 5 days. The current study evaluated the in vivo use of these stored viable peripheral nerve allografts. We hypothesized that stored viable peripheral nerve allografts with viable axons would enable successful in vivo repair of segmental-loss peripheral nerve injuries via polyethylene glycol-fusion. Polyethylene glycol-fused viable peripheral nerve allografts were classified as successful if they produced significantly improved locomotor recovery, as evaluated by the sciatic functional index, within 8 weeks post-repair. Many Sprague–Dawley and Lewis rats with successfully polyethylene glycol-fused viable peripheral nerve allografts had significantly improved sciatic functional index scores beginning at 5 weeks post-operatively. There was no significant difference in the efficiency and extent of successful polyethylene glycol fusion between stored and freshly harvested viable peripheral nerve allografts. In contrast, rats with non-fused negative control viable peripheral nerve allografts showed no recovery by 8 weeks post-operatively. Additional confirmatory outcome measures included in vivo compound action potentials and assessments of axon morphometry. These results suggest that viable peripheral nerve allografts can be stored and later used for successful polyethylene glycol fusion repair of segmental-loss peripheral nerve injuries.

Key words: axonal morphometrics, axotomy, Lewis, peripheral nerve injury, peripheral nerve repair, polyethylene glycol fusion, sciatic nerve ablation, Sprague–Dawley, tissue storage solutions